Assay Detail
ADMET
CHEMBL3994300
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Effect on ATP homeostasis in human HCT116 cells assessed as reduction in ATP level after 3 hrs in absence of glucose by ATP bioluminescent assay
12
Total Activities
6
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | ADMET |
| Organism | Homo sapiens |
| Cell Type | HCT-116 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Benzimidazole inhibitors from the Niclosamide chemotype inhibit Wnt/β-catenin signaling with selectivity over effects on ATP homeostasis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 6.40 | 7.25 |
| Activity | 4 | - | - |
| IC50 | 4 | 5.91 | 6.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1448 | NICLOSAMIDE | 4.0 | 3 | 7.25 |
| CHEMBL1944805 | — | — | 2 | 5.54 |
| CHEMBL4102601 | — | — | 3 | 5.22 |
| CHEMBL4063414 | — | — | 2 | - |
| CHEMBL1944796 | — | — | 1 | - |
| CHEMBL4087103 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1448 | NICLOSAMIDE | EC50 | = | 56.0 | nM | 7.25 |
| CHEMBL1448 | NICLOSAMIDE | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL1944805 | — | EC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL4102601 | — | IC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL4063414 | — | EC50 | >= | 5000.0 | nM | - |
| CHEMBL4102601 | — | EC50 | > | 5000.0 | nM | - |
| CHEMBL1944805 | — | Activity | - | — | - | |
| CHEMBL4063414 | — | Activity | - | — | - | |
| CHEMBL4102601 | — | Activity | - | — | - | |
| CHEMBL1448 | NICLOSAMIDE | Activity | - | — | - | |
| CHEMBL1944796 | — | IC50 | < | 250.0 | nM | - |
| CHEMBL4087103 | — | IC50 | < | 250.0 | nM | - |