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Assay Detail

CHEMBL3995155

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human placental microsomal 17beta-HSD2 using [2,4,6,7-3H]-E2 as substrate after 20 mins in presence of NAD+ by RP-HPLC method
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

17-beta-hydroxysteroid dehydrogenase type 2 (CHEMBL2789)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Treatment of estrogen-dependent diseases: Design, synthesis and profiling of a selective 17β-HSD1 inhibitor with sub-nanomolar IC50 for a proof-of-principle study.
Abdelsamie AS, van Koppen CJ, Bey E, Salah M, Börger C, Siebenbürger L, Laschke MW, Menger MD, Frotscher M.
Eur J Med Chem (2017) 127 : 944 -957
PubMed 27852458 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 7.51 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4092593 1 8.85
CHEMBL4063985 1 8.19
CHEMBL4101264 1 8.15
CHEMBL4103524 1 8.14
CHEMBL4082298 1 8.05
CHEMBL4080385 1 8.05
CHEMBL4099360 1 8.00
CHEMBL4060257 1 7.96
CHEMBL4062959 1 7.96
CHEMBL4077264 1 7.75
CHEMBL4073469 1 7.72
CHEMBL4063839 1 7.70
CHEMBL4062685 1 7.68
CHEMBL4077041 1 7.58
CHEMBL4091053 1 7.58
CHEMBL4088861 1 7.54
CHEMBL4061631 1 7.54
CHEMBL4083849 1 7.46
CHEMBL4072426 1 7.42
CHEMBL4091749 1 7.41
CHEMBL4091622 1 7.41
CHEMBL4079675 1 7.39
CHEMBL4101849 1 7.33
CHEMBL4071808 1 7.26
CHEMBL4071588 1 7.15
CHEMBL4067584 1 7.07
CHEMBL4094112 1 7.07
CHEMBL4084832 1 6.85
CHEMBL4095004 1 6.75
CHEMBL4089862 1 6.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4092593 IC50 = 1.4 nM 8.85
CHEMBL4063985 IC50 = 6.5 nM 8.19
CHEMBL4101264 IC50 = 7.0 nM 8.15
CHEMBL4103524 IC50 = 7.2 nM 8.14
CHEMBL4082298 IC50 = 9.0 nM 8.05
CHEMBL4080385 IC50 = 9.0 nM 8.05
CHEMBL4099360 IC50 = 10.0 nM 8.00
CHEMBL4060257 IC50 = 11.0 nM 7.96
CHEMBL4062959 IC50 = 11.0 nM 7.96
CHEMBL4077264 IC50 = 18.0 nM 7.75
CHEMBL4073469 IC50 = 19.0 nM 7.72
CHEMBL4063839 IC50 = 20.0 nM 7.70
CHEMBL4062685 IC50 = 21.0 nM 7.68
CHEMBL4077041 IC50 = 26.0 nM 7.58
CHEMBL4091053 IC50 = 26.0 nM 7.58
CHEMBL4061631 IC50 = 29.0 nM 7.54
CHEMBL4088861 IC50 = 29.0 nM 7.54
CHEMBL4083849 IC50 = 35.0 nM 7.46
CHEMBL4072426 IC50 = 38.0 nM 7.42
CHEMBL4091749 IC50 = 39.0 nM 7.41
CHEMBL4091622 IC50 = 39.0 nM 7.41
CHEMBL4079675 IC50 = 41.0 nM 7.39
CHEMBL4101849 IC50 = 47.0 nM 7.33
CHEMBL4071808 IC50 = 55.0 nM 7.26
CHEMBL4071588 IC50 = 71.0 nM 7.15
CHEMBL4067584 IC50 = 86.0 nM 7.07
CHEMBL4094112 IC50 = 85.0 nM 7.07
CHEMBL4084832 IC50 = 140.0 nM 6.85
CHEMBL4095004 IC50 = 180.0 nM 6.75
CHEMBL4089862 IC50 = 250.0 nM 6.60
CHEMBL4104610 IC50 = 300.0 nM 6.52
CHEMBL4083262 IC50 = 540.0 nM 6.27