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Assay Detail

CHEMBL4001045

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human N-terminal HA-tagged GPR35a expressed in human U2OS cells co-expressing GFP-fused beta arrestin-2 assessed as inhibition of agonist induced redistribution of GFP-fused beta arrestin-2 preincubated for 15 mins followed by agonist addition by fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL1293267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationships of benzothiazole GPR35 antagonists.
Abdalhameed MM, Zhao P, Hurst DP, Reggio PH, Abood ME, Croatt MP.
Bioorg Med Chem Lett (2017) 27 : 612 -615
PubMed 27989666 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 6.26 6.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1368962 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1368962 EC50 = 550.0 nM 6.26