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Assay Detail

CHEMBL4003206

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human spleen NAAA expressed in HEK293 cells using PAMCA as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

N-acylethanolamine-hydrolyzing acid amidase (CHEMBL4349)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Progress in the development of β-lactams as N-Acylethanolamine Acid Amidase (NAAA) inhibitors: Synthesis and SAR study of new, potent N-O-substituted derivatives.
Petracca R, Ponzano S, Bertozzi SM, Sasso O, Piomelli D, Bandiera T, Bertozzi F.
Eur J Med Chem (2017) 126 : 561 -575
PubMed 27915171 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.47 6.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3770509 1 6.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3770509 IC50 = 340.0 nM 6.47