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Assay Detail

CHEMBL4003553

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of GSK3103956A from human BRD4 (1 to 477 residues) BD1 Y390A mutant incubated for 30 mins in dark by TR-FRET assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent, Cell Penetrant, and Selective p300/CBP-Associated Factor (PCAF)/General Control Nonderepressible 5 (GCN5) Bromodomain Chemical Probe.
Humphreys PG, Bamborough P, Chung CW, Craggs PD, Gordon L, Grandi P, Hayhow TG, Hussain J, Jones KL, Lindon M, Michon AM, Renaux JF, Suckling CJ, Tough DF, Prinjha RK.
J Med Chem (2017) 60 : 695 -709
PubMed 28002667 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 4.68 4.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4085471 1 4.80
CHEMBL4096572 1 4.80
CHEMBL4074202 1 4.70
CHEMBL4095646 1 4.70
CHEMBL4075248 1 4.60
CHEMBL4065579 1 4.50
CHEMBL4063947 1 -
CHEMBL4091717 1 -
CHEMBL4101945 1 -
CHEMBL4101756 1 -
CHEMBL4071581 1 -
CHEMBL4093233 1 -
CHEMBL4100959 1 -
CHEMBL4096641 1 -
CHEMBL4069412 1 -
CHEMBL4061691 1 -
CHEMBL4084253 1 -
CHEMBL4083019 1 -
CHEMBL4061142 1 -
CHEMBL4099456 1 -
CHEMBL4072538 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4085471 IC50 = 15848.93 nM 4.80
CHEMBL4096572 IC50 = 15848.93 nM 4.80
CHEMBL4074202 IC50 = 19952.62 nM 4.70
CHEMBL4095646 IC50 = 19952.62 nM 4.70
CHEMBL4075248 IC50 = 25118.86 nM 4.60
CHEMBL4065579 IC50 = 31622.78 nM 4.50
CHEMBL4063947 IC50 > 50118.72 nM -
CHEMBL4091717 IC50 > 50118.72 nM -
CHEMBL4101945 IC50 > 50118.72 nM -
CHEMBL4101756 IC50 > 50118.72 nM -
CHEMBL4071581 IC50 > 50118.72 nM -
CHEMBL4093233 IC50 = 125892.54 nM -
CHEMBL4100959 IC50 > 50118.72 nM -
CHEMBL4096641 IC50 > 50118.72 nM -
CHEMBL4069412 IC50 > 50118.72 nM -
CHEMBL4061691 IC50 > 50118.72 nM -
CHEMBL4084253 IC50 > 50118.72 nM -
CHEMBL4083019 IC50 > 50118.72 nM -
CHEMBL4061142 IC50 > 50118.72 nM -
CHEMBL4099456 IC50 > 50118.72 nM -
CHEMBL4072538 IC50 > 50118.72 nM -