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Assay Detail

CHEMBL4006066

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ATX expressed in HEK293 Flp-In cells assessed as decrease in choline release from LPC measured every 30 secs for 90 mins by HVA based fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Autotaxin (CHEMBL3691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational Design of Autotaxin Inhibitors by Structural Evolution of Endogenous Modulators.
Keune WJ, Potjewyd F, Heidebrecht T, Salgado-Polo F, Macdonald SJ, Chelvarajan L, Abdel Latif A, Soman S, Morris AJ, Watson AJ, Jamieson C, Perrakis A.
J Med Chem (2017) 60 : 2006 -2017
PubMed 28165241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.32 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4062929 1 7.70
CHEMBL4093316 1 7.00
CHEMBL4081367 1 6.89
CHEMBL4105619 1 6.70
CHEMBL4090866 1 6.68
CHEMBL4071554 1 6.50
CHEMBL4094214 1 6.30
CHEMBL4070768 1 6.25
CHEMBL4098586 1 6.14
CHEMBL4085570 1 6.09
CHEMBL4101049 1 6.00
CHEMBL4083075 1 5.68
CHEMBL4072603 1 5.60
CHEMBL272427 TAURURSODIOL 4.0 1 4.99
CHEMBL4064007 1 -
CHEMBL4078911 1 -
CHEMBL4096938 1 -
CHEMBL4097881 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4062929 IC50 = 20.0 nM 7.70
CHEMBL4093316 IC50 = 100.0 nM 7.00
CHEMBL4081367 IC50 = 129.0 nM 6.89
CHEMBL4105619 IC50 = 202.0 nM 6.70
CHEMBL4090866 IC50 = 208.0 nM 6.68
CHEMBL4071554 IC50 = 313.0 nM 6.50
CHEMBL4094214 IC50 = 500.0 nM 6.30
CHEMBL4070768 IC50 = 563.0 nM 6.25
CHEMBL4098586 IC50 = 717.0 nM 6.14
CHEMBL4085570 IC50 = 814.0 nM 6.09
CHEMBL4101049 IC50 = 1000.0 nM 6.00
CHEMBL4083075 IC50 = 2100.0 nM 5.68
CHEMBL4072603 IC50 = 2500.0 nM 5.60
CHEMBL272427 TAURURSODIOL IC50 = 10300.0 nM 4.99
CHEMBL4064007 IC50 > 30000.0 nM -
CHEMBL4078911 IC50 > 30000.0 nM -
CHEMBL4096938 IC50 > 30000.0 nM -
CHEMBL4097881 IC50 > 30000.0 nM -