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Assay Detail

CHEMBL4008250

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against Hepatitis C virus genotype 1b infected in human HuH7 cells preincubated with cells for 3 days followed by viral infection by luciferase assay
14
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Hepacivirus hominis
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatitis C virus (CHEMBL379)
Type ORGANISM
Organism Hepacivirus hominis

Publication

Discovery and Synthesis of a Phosphoramidate Prodrug of a Pyrrolo[2,1-f][triazin-4-amino] Adenine C-Nucleoside (GS-5734) for the Treatment of Ebola and Emerging Viruses.
Siegel D, Hui HC, Doerffler E, Clarke MO, Chun K, Zhang L, Neville S, Carra E, Lew W, Ross B, Wang Q, Wolfe L, Jordan R, Soloveva V, Knox J, Perry J, Perron M, Stray KM, Barauskas O, Feng JY, Xu Y, Lee G, Rheingold AL, Ray AS, Bannister R, Strickley R, Swaminathan S, Lee WA, Bavari S, Cihlar T, Lo MK, Warren TK, Mackman RL.
J Med Chem (2017) 60 : 1648 -1661
PubMed 28124907 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 6.18 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4079082 1 8.46
CHEMBL4065616 REMDESIVIR 4.0 2 7.64
CHEMBL3120793 GS-6620 1.0 1 6.51
CHEMBL3120789 1 6.43
CHEMBL4069270 1 5.60
CHEMBL2016757 GS-441524 1.0 1 5.39
CHEMBL4086934 1 5.16
CHEMBL4090316 1 4.92
CHEMBL2016758 1 4.42
CHEMBL2016760 1 -
CHEMBL4065501 1 -
CHEMBL4066445 1 -
CHEMBL3120779 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4079082 EC50 = 3.5 nM 8.46
CHEMBL4065616 REMDESIVIR EC50 = 23.0 nM 7.64
CHEMBL4065616 REMDESIVIR EC50 = 57.0 nM 7.24
CHEMBL3120793 GS-6620 EC50 = 310.0 nM 6.51
CHEMBL3120789 EC50 = 370.0 nM 6.43
CHEMBL4069270 EC50 = 2500.0 nM 5.60
CHEMBL2016757 GS-441524 EC50 = 4100.0 nM 5.39
CHEMBL4086934 EC50 = 6900.0 nM 5.16
CHEMBL4090316 EC50 = 12000.0 nM 4.92
CHEMBL2016758 EC50 = 38000.0 nM 4.42
CHEMBL2016760 EC50 > 88000.0 nM -
CHEMBL4065501 EC50 > 44000.0 nM -
CHEMBL4066445 EC50 > 44000.0 nM -
CHEMBL3120779 EC50 > 44000.0 nM -