Assay Detail
Binding
CHEMBL4008437
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human complement factor D catalytic domain (G24 to A253 residues) expressed in Escherichia coli Rosetta using Z-Lys-thiobenzyl as substrate pretreated for 1 hr followed by substrate addition by 2,4-dinitrobenzenesulfonyl-2,7-desmethyl-fluorescein probe based spectrofluorimetric thioesterolysis assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structure-Based Library Design and Fragment Screening for the Identification of Reversible Complement Factor D Protease Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.81 | 4.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4076148 | — | — | 1 | 4.85 |
| CHEMBL4093577 | — | — | 1 | 4.77 |
| CHEMBL4065974 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4076148 | — | IC50 | = | 14000.0 | nM | 4.85 |
| CHEMBL4093577 | — | IC50 | = | 17000.0 | nM | 4.77 |
| CHEMBL4065974 | — | IC50 | > | 30000.0 | nM | - |