Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4013155

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-fused EGFR L858R mutant (669 to 1210 residues) expressed in baculovirus using TK-substrate-biotin preincubated for 30 mins followed by substrate addition measured after 15 mins by HTFR assay
44
Total Activities
44
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trisubstituted Pyridinylimidazoles as Potent Inhibitors of the Clinically Resistant L858R/T790M/C797S EGFR Mutant: Targeting of Both Hydrophobic Regions and the Phosphate Binding Site.
Günther M, Lategahn J, Juchum M, Döring E, Keul M, Engel J, Tumbrink HL, Rauh D, Laufer S.
J Med Chem (2017) 60 : 5613 -5637
PubMed 28603991 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 44 8.04 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4064942 1 9.00
CHEMBL4091569 1 8.96
CHEMBL3353410 OSIMERTINIB 4.0 1 8.89
CHEMBL4062543 1 8.77
CHEMBL4071180 1 8.72
CHEMBL4070707 1 8.72
CHEMBL4071012 1 8.66
CHEMBL321494 1 8.60
CHEMBL4081860 1 8.59
CHEMBL4061473 1 8.51
CHEMBL4102224 1 8.49
CHEMBL4089279 1 8.31
CHEMBL4076854 1 8.25
CHEMBL4102577 1 8.17
CHEMBL4086878 1 8.00
CHEMBL4076363 1 7.92
CHEMBL4082884 1 7.80
CHEMBL4089601 1 7.66
CHEMBL4086078 1 7.60
CHEMBL4081732 1 7.40
CHEMBL4068092 1 7.34
CHEMBL4062722 1 7.26
CHEMBL4067255 1 7.12
CHEMBL4092003 1 7.12
CHEMBL4084248 1 6.84
CHEMBL4101193 1 6.44
CHEMBL1173655 AFATINIB 4.0 1 -
CHEMBL4087268 1 -
CHEMBL4079393 1 -
CHEMBL4098072 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4064942 IC50 = 1.0 nM 9.00
CHEMBL4091569 IC50 = 1.1 nM 8.96
CHEMBL3353410 OSIMERTINIB IC50 = 1.3 nM 8.89
CHEMBL4062543 IC50 = 1.7 nM 8.77
CHEMBL4071180 IC50 = 1.9 nM 8.72
CHEMBL4070707 IC50 = 1.9 nM 8.72
CHEMBL4071012 IC50 = 2.2 nM 8.66
CHEMBL321494 IC50 = 2.5 nM 8.60
CHEMBL4081860 IC50 = 2.6 nM 8.59
CHEMBL4061473 IC50 = 3.1 nM 8.51
CHEMBL4102224 IC50 = 3.2 nM 8.49
CHEMBL4089279 IC50 = 4.9 nM 8.31
CHEMBL4076854 IC50 = 5.6 nM 8.25
CHEMBL4102577 IC50 = 6.8 nM 8.17
CHEMBL4086878 IC50 = 10.0 nM 8.00
CHEMBL4076363 IC50 = 12.0 nM 7.92
CHEMBL4082884 IC50 = 16.0 nM 7.80
CHEMBL4089601 IC50 = 22.0 nM 7.66
CHEMBL4086078 IC50 = 25.0 nM 7.60
CHEMBL4081732 IC50 = 40.0 nM 7.40
CHEMBL4068092 IC50 = 46.0 nM 7.34
CHEMBL4062722 IC50 = 55.0 nM 7.26
CHEMBL4067255 IC50 = 75.0 nM 7.12
CHEMBL4092003 IC50 = 76.0 nM 7.12
CHEMBL4084248 IC50 = 144.0 nM 6.84
CHEMBL4101193 IC50 = 360.0 nM 6.44
CHEMBL1229592 IC50 < 1.0 nM -
CHEMBL4060003 IC50 < 1.0 nM -
CHEMBL4071368 IC50 < 1.0 nM -
CHEMBL4098412 IC50 < 1.0 nM -
CHEMBL4090686 IC50 < 1.0 nM -
CHEMBL4100860 IC50 < 1.0 nM -
CHEMBL4082362 IC50 < 1.0 nM -
CHEMBL4100377 IC50 < 1.0 nM -
CHEMBL939 GEFITINIB IC50 < 1.0 nM -
CHEMBL4105526 IC50 < 1.0 nM -
CHEMBL4078963 IC50 < 1.0 nM -
CHEMBL4096980 IC50 < 1.0 nM -
CHEMBL4100777 IC50 < 1.0 nM -
CHEMBL4094927 IC50 < 1.0 nM -
CHEMBL4098072 IC50 < 1.0 nM -
CHEMBL4079393 IC50 < 1.0 nM -
CHEMBL4087268 IC50 < 1.0 nM -
CHEMBL1173655 AFATINIB IC50 < 1.0 nM -