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Assay Detail

CHEMBL4013164

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human EGFR T790M/C797S/L858R triple mutant preincubated for 20 mins followed by [33P]ATP addition measured after 2 hrs
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trisubstituted Pyridinylimidazoles as Potent Inhibitors of the Clinically Resistant L858R/T790M/C797S EGFR Mutant: Targeting of Both Hydrophobic Regions and the Phosphate Binding Site.
Günther M, Lategahn J, Juchum M, Döring E, Keul M, Engel J, Tumbrink HL, Rauh D, Laufer S.
J Med Chem (2017) 60 : 5613 -5637
PubMed 28603991 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.14 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4090686 1 8.15
CHEMBL4100860 1 8.10
CHEMBL4071368 1 7.96
CHEMBL4060003 1 7.28
CHEMBL4098072 1 7.05
CHEMBL4094927 1 6.78
CHEMBL4102577 1 6.65
CHEMBL3353410 OSIMERTINIB 4.0 1 6.56
CHEMBL1229592 1 5.69
CHEMBL4087268 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4090686 IC50 = 7.0 nM 8.15
CHEMBL4100860 IC50 = 8.0 nM 8.10
CHEMBL4071368 IC50 = 11.0 nM 7.96
CHEMBL4060003 IC50 = 53.0 nM 7.28
CHEMBL4098072 IC50 = 90.0 nM 7.05
CHEMBL4094927 IC50 = 167.0 nM 6.78
CHEMBL4102577 IC50 = 223.0 nM 6.65
CHEMBL3353410 OSIMERTINIB IC50 = 278.0 nM 6.56
CHEMBL1229592 IC50 = 2053.0 nM 5.69
CHEMBL4087268 IC50 - -