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Assay Detail

CHEMBL4013166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human N-terminal GST-fused EGFR cytoplasmic domain (669 to 1210 residues) expressed in baculovirus using TK-substrate-biotin incubated for 2 to 90 mins by HTFR assay
8
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trisubstituted Pyridinylimidazoles as Potent Inhibitors of the Clinically Resistant L858R/T790M/C797S EGFR Mutant: Targeting of Both Hydrophobic Regions and the Phosphate Binding Site.
Günther M, Lategahn J, Juchum M, Döring E, Keul M, Engel J, Tumbrink HL, Rauh D, Laufer S.
J Med Chem (2017) 60 : 5613 -5637
PubMed 28603991 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 9.07 10.15
Kinact 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4100860 2 10.15
CHEMBL4098072 2 9.46
CHEMBL4071012 2 8.80
CHEMBL3353410 OSIMERTINIB 4.0 2 7.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4100860 Ki = 0.07 nM 10.15
CHEMBL4098072 Ki = 0.35 nM 9.46
CHEMBL4071012 Ki = 1.6 nM 8.80
CHEMBL3353410 OSIMERTINIB Ki = 14.0 nM 7.85
CHEMBL4071012 Kinact = 0.09 /min -
CHEMBL3353410 OSIMERTINIB Kinact = 0.43 /min -
CHEMBL4098072 Kinact = 0.16 /min -
CHEMBL4100860 Kinact = 0.16 /min -