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Assay Detail

CHEMBL4013829

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FGFR1 in HUVEC assessed as reduction in FGF2-induced ERK phosphorylation preincubated for 60 mins followed by FGF2 stimulation for 10 mins by AlphaScreen assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 1 (CHEMBL3650)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the Irreversible Covalent FGFR Inhibitor 8-(3-(4-Acryloylpiperazin-1-yl)propyl)-6-(2,6-dichloro-3,5-dimethoxyphenyl)-2-(methylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (PRN1371) for the Treatment of Solid Tumors.
Brameld KA, Owens TD, Verner E, Venetsanakos E, Bradshaw JM, Phan VT, Tam D, Leung K, Shu J, LaStant J, Loughhead DG, Ton T, Karr DE, Gerritsen ME, Goldstein DM, Funk JO.
J Med Chem (2017) 60 : 6516 -6527
PubMed 28665128 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.28 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4068509 PRN-1371 1.0 1 8.96
CHEMBL4099992 1 8.92
CHEMBL4089842 1 8.89
CHEMBL4066763 1 8.80
CHEMBL4061286 1 8.80
CHEMBL4088057 1 8.66
CHEMBL4092456 1 8.62
CHEMBL4099578 1 8.47
CHEMBL4095844 1 8.39
CHEMBL4061766 1 8.25
CHEMBL4095783 1 8.22
CHEMBL4081984 1 8.11
CHEMBL4064081 1 8.07
CHEMBL4102642 1 8.03
CHEMBL4083151 1 8.02
CHEMBL4084708 1 7.96
CHEMBL4062003 1 7.96
CHEMBL4064756 1 7.92
CHEMBL4073347 1 7.80
CHEMBL4102058 1 7.55
CHEMBL4100201 1 7.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4068509 PRN-1371 IC50 = 1.1 nM 8.96
CHEMBL4099992 IC50 = 1.2 nM 8.92
CHEMBL4089842 IC50 = 1.3 nM 8.89
CHEMBL4066763 IC50 = 1.6 nM 8.80
CHEMBL4061286 IC50 = 1.6 nM 8.80
CHEMBL4088057 IC50 = 2.2 nM 8.66
CHEMBL4092456 IC50 = 2.4 nM 8.62
CHEMBL4099578 IC50 = 3.4 nM 8.47
CHEMBL4095844 IC50 = 4.1 nM 8.39
CHEMBL4061766 IC50 = 5.6 nM 8.25
CHEMBL4095783 IC50 = 6.0 nM 8.22
CHEMBL4081984 IC50 = 7.8 nM 8.11
CHEMBL4064081 IC50 = 8.6 nM 8.07
CHEMBL4102642 IC50 = 9.4 nM 8.03
CHEMBL4083151 IC50 = 9.5 nM 8.02
CHEMBL4084708 IC50 = 11.0 nM 7.96
CHEMBL4062003 IC50 = 11.0 nM 7.96
CHEMBL4064756 IC50 = 12.0 nM 7.92
CHEMBL4073347 IC50 = 16.0 nM 7.80
CHEMBL4102058 IC50 = 28.0 nM 7.55
CHEMBL4100201 IC50 = 37.0 nM 7.43