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Assay Detail

CHEMBL4014420

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged PTP1B (1 to 321 residues) (unknown origin) using p-NPP as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Phloroglucinol Derivatives with Protein Tyrosine Phosphatase 1B Inhibitory Activities from Eugenia jambolana Seeds.
Liu F, Yuan T, Liu W, Ma H, Seeram NP, Li Y, Xu L, Mu Y, Huang X, Li L.
J Nat Prod (2017) 80 : 544 -550
PubMed 28134521 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.79 6.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4095230 1 6.38
CHEMBL4087528 1 6.35
CHEMBL4067813 1 5.80
CHEMBL4086544 1 5.75
CHEMBL4102979 1 5.70
CHEMBL1232829 1 5.70
CHEMBL4078699 1 5.57
CHEMBL169 URSOLIC ACID 2.0 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4095230 IC50 = 420.0 nM 6.38
CHEMBL4087528 IC50 = 450.0 nM 6.35
CHEMBL4067813 IC50 = 1600.0 nM 5.80
CHEMBL4086544 IC50 = 1800.0 nM 5.75
CHEMBL4102979 IC50 = 2000.0 nM 5.70
CHEMBL1232829 IC50 = 2000.0 nM 5.70
CHEMBL4078699 IC50 = 2700.0 nM 5.57
CHEMBL169 URSOLIC ACID IC50 = 8900.0 nM 5.05