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Assay Detail

CHEMBL4014442

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiosteoporotic activity in mouse RAW264.7 cells assessed as inhibition of RANKL-induced osteoclast differentiation after 4 to 5 days by TRAP assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Anti-inflammatory and Antiosteoporosis Flavonoids from the Rhizomes of Helminthostachys zeylanica.
Huang YL, Shen CC, Shen YC, Chiou WF, Chen CC.
J Nat Prod (2017) 80 : 246 -253
PubMed 28169537 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.43 5.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4088548 1 5.75
CHEMBL560314 UGONIN M 1 5.60
CHEMBL4096267 1 5.50
CHEMBL550505 UGONIN O 1 5.48
CHEMBL4088204 1 5.46
CHEMBL4066932 1 5.35
CHEMBL2308661 1 5.34
CHEMBL44 GENISTEIN 2.0 1 4.94
CHEMBL4090988 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4088548 IC50 = 1800.0 nM 5.75
CHEMBL560314 UGONIN M IC50 = 2500.0 nM 5.60
CHEMBL4096267 IC50 = 3200.0 nM 5.50
CHEMBL550505 UGONIN O IC50 = 3300.0 nM 5.48
CHEMBL4088204 IC50 = 3500.0 nM 5.46
CHEMBL4066932 IC50 = 4500.0 nM 5.35
CHEMBL2308661 IC50 = 4600.0 nM 5.34
CHEMBL44 GENISTEIN IC50 = 11400.0 nM 4.94
CHEMBL4090988 IC50 > 10000.0 nM -