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Assay Detail

CHEMBL4016543

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kdelta in human whole blood assessed as reduction in anti-IgD-mediated B-cell activation by measuring decrease in CD19+ cells expressing CD69 preincubated for 1 hr followed by IgD activation measured after overnight incubation
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification of a Potent, Selective, and Efficacious Phosphatidylinositol 3-Kinase δ (PI3Kδ) Inhibitor for the Treatment of Immunological Disorders.
Liu Q, Shi Q, Marcoux D, Batt DG, Cornelius L, Qin LY, Ruan Z, Neels J, Beaudoin-Bertrand M, Srivastava AS, Li L, Cherney RJ, Gong H, Watterson SH, Weigelt C, Gillooly KM, McIntyre KW, Xie JH, Obermeier MT, Fura A, Sleczka B, Stefanski K, Fancher RM, Padmanabhan S, Rp T, Kundu I, Rajareddy K, Smith R, Hennan JK, Xing D, Fan J, Levesque PC, Ruan Q, Pitt S, Zhang R, Pedicord D, Pan J, Yarde M, Lu H, Lippy J, Goldstine C, Skala S, Rampulla RA, Mathur A, Gupta A, Arunachalam PN, Sack JS, Muckelbauer JK, Cvijic ME, Salter-Cid LM, Bhide RS, Poss MA, Hynes J, Carter PH, Macor JE, Ruepp S, Schieven GL, Tino JA.
J Med Chem (2017) 60 : 5193 -5208
PubMed 28541707 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 7.08 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4068514 1 8.15
CHEMBL4092344 1 7.80
CHEMBL4071965 1 7.75
CHEMBL4105075 1 7.62
CHEMBL4087215 1 7.51
CHEMBL4102552 1 7.51
CHEMBL4093883 1 7.46
CHEMBL4064666 1 7.46
CHEMBL4092240 1 7.44
CHEMBL4081766 1 7.42
CHEMBL4067315 1 7.42
CHEMBL4066316 1 7.41
CHEMBL4068408 1 7.38
CHEMBL4059848 1 7.24
CHEMBL4095752 1 7.18
CHEMBL4064543 1 7.14
CHEMBL4102444 1 7.13
CHEMBL4073255 1 7.10
CHEMBL4074315 1 7.05
CHEMBL4100099 1 7.04
CHEMBL4086128 1 7.02
CHEMBL4091840 1 7.00
CHEMBL4083358 1 6.98
CHEMBL4074193 1 6.85
CHEMBL4084604 1 6.82
CHEMBL4076137 1 6.80
CHEMBL4078237 1 6.66
CHEMBL4070029 1 6.64
CHEMBL4094814 1 6.64
CHEMBL4094693 1 6.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4068514 IC50 = 7.0 nM 8.15
CHEMBL4092344 IC50 = 16.0 nM 7.80
CHEMBL4071965 IC50 = 18.0 nM 7.75
CHEMBL4105075 IC50 = 24.0 nM 7.62
CHEMBL4087215 IC50 = 31.0 nM 7.51
CHEMBL4102552 IC50 = 31.0 nM 7.51
CHEMBL4093883 IC50 = 35.0 nM 7.46
CHEMBL4064666 IC50 = 35.0 nM 7.46
CHEMBL4092240 IC50 = 36.0 nM 7.44
CHEMBL4067315 IC50 = 38.0 nM 7.42
CHEMBL4081766 IC50 = 38.0 nM 7.42
CHEMBL4066316 IC50 = 39.0 nM 7.41
CHEMBL4068408 IC50 = 42.0 nM 7.38
CHEMBL4059848 IC50 = 58.0 nM 7.24
CHEMBL4095752 IC50 = 66.0 nM 7.18
CHEMBL4064543 IC50 = 72.0 nM 7.14
CHEMBL4102444 IC50 = 74.0 nM 7.13
CHEMBL4073255 IC50 = 79.0 nM 7.10
CHEMBL4074315 IC50 = 90.0 nM 7.05
CHEMBL4100099 IC50 = 92.0 nM 7.04
CHEMBL4086128 IC50 = 95.0 nM 7.02
CHEMBL4091840 IC50 = 100.0 nM 7.00
CHEMBL4083358 IC50 = 105.0 nM 6.98
CHEMBL4074193 IC50 = 140.0 nM 6.85
CHEMBL4084604 IC50 = 150.0 nM 6.82
CHEMBL4076137 IC50 = 160.0 nM 6.80
CHEMBL4078237 IC50 = 220.0 nM 6.66
CHEMBL4094814 IC50 = 230.0 nM 6.64
CHEMBL4070029 IC50 = 230.0 nM 6.64
CHEMBL4094693 IC50 = 280.0 nM 6.55
CHEMBL4097222 IC50 = 370.0 nM 6.43
CHEMBL4070164 IC50 = 410.0 nM 6.39
CHEMBL4087001 IC50 = 500.0 nM 6.30
CHEMBL4096288 IC50 = 550.0 nM 6.26
CHEMBL4104044 IC50 = 570.0 nM 6.24
CHEMBL4088567 IC50 - -
CHEMBL4084490 IC50 - -