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Assay Detail

CHEMBL4018933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]CGS21680 from human A2A adenosine receptor expressed in HEK293 cell membranes after 60 mins by gamma counting method
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Polypharmacology of N6-(3-Iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) and Related A3 Adenosine Receptor Ligands: Peroxisome Proliferator Activated Receptor (PPAR) γ Partial Agonist and PPARδ Antagonist Activity Suggests Their Antidiabetic Potential.
Yu J, Ahn S, Kim HJ, Lee M, Ahn S, Kim J, Jin SH, Lee E, Kim G, Cheong JH, Jacobson KA, Jeong LS, Noh M.
J Med Chem (2017) 60 : 7459 -7475
PubMed 28799755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 6.38 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL596014 1 7.82
CHEMBL596042 1 7.24
CHEMBL594625 1 6.91
CHEMBL200732 1 6.65
CHEMBL413686 1 6.47
CHEMBL500569 1 6.36
CHEMBL522152 1 6.32
CHEMBL227279 1 5.80
CHEMBL603424 1 5.80
CHEMBL119709 IB-MECA 1 5.54
CHEMBL431733 NAMODENOSON 3.0 1 5.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL596014 Ki = 15.2 nM 7.82
CHEMBL596042 Ki = 57.6 nM 7.24
CHEMBL594625 Ki = 122.0 nM 6.91
CHEMBL200732 Ki = 223.0 nM 6.65
CHEMBL413686 Ki = 341.0 nM 6.47
CHEMBL500569 Ki = 440.0 nM 6.36
CHEMBL522152 Ki = 475.0 nM 6.32
CHEMBL227279 Ki = 1600.0 nM 5.80
CHEMBL603424 Ki = 1600.0 nM 5.80
CHEMBL119709 IB-MECA Ki = 2910.0 nM 5.54
CHEMBL431733 NAMODENOSON Ki = 5360.0 nM 5.27