Assay Detail
Binding
CHEMBL4020035
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive inhibition of 6xHis-tagged human liver glycogen phosphorylase-a expressed in Escherichia coli BL21 Gold (DE3) assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen preincubated for 15 mins with AMP and glycogen followed by glucose-1-phosphate addition by Lineweaver-Burk plot method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Nanomolar Inhibitors of Glycogen Phosphorylase Based on β-d-Glucosaminyl Heterocycles: A Combined Synthetic, Enzyme Kinetic, and Protein Crystallography Study.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.51 | 6.84 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4101331 | — | — | 1 | 6.84 |
| CHEMBL4095486 | — | — | 1 | 5.56 |
| CHEMBL4068103 | — | — | 1 | 5.12 |
| CHEMBL4087784 | — | — | 1 | 4.51 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4101331 | — | Ki | = | 143.0 | nM | 6.84 |
| CHEMBL4095486 | — | Ki | = | 2730.0 | nM | 5.56 |
| CHEMBL4068103 | — | Ki | = | 7600.0 | nM | 5.12 |
| CHEMBL4087784 | — | Ki | = | 30800.0 | nM | 4.51 |