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Assay Detail

CHEMBL4020042

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to biotin labelled human EED (78 to 441 residues) by SPR assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polycomb protein EED (CHEMBL2189117)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Are We There Yet? Applying Thermodynamic and Kinetic Profiling on Embryonic Ectoderm Development (EED) Hit-to-Lead Program.
Wang Y, Edalji RP, Panchal SC, Sun C, Djuric SW, Vasudevan A.
J Med Chem (2017) 60 : 8321 -8335
PubMed 28926243 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 21 6.51 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4084398 1 9.00
CHEMBL4104741 1 8.70
CHEMBL4060827 1 8.40
CHEMBL4081496 1 7.70
CHEMBL4102320 1 7.37
CHEMBL4100850 1 7.21
CHEMBL4071358 1 7.00
CHEMBL4072980 1 6.85
CHEMBL4075197 1 6.82
CHEMBL4077899 1 6.70
CHEMBL4078052 1 6.68
CHEMBL4092154 1 6.54
CHEMBL4079004 1 6.52
CHEMBL4093678 1 5.85
CHEMBL4103307 1 5.58
CHEMBL4067115 1 5.51
CHEMBL4085375 1 5.32
CHEMBL4093134 1 5.01
CHEMBL4074086 1 4.87
CHEMBL51594 1 4.66
CHEMBL4074159 1 4.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4084398 Kd = 1.0 nM 9.00
CHEMBL4104741 Kd = 2.0 nM 8.70
CHEMBL4060827 Kd = 4.0 nM 8.40
CHEMBL4081496 Kd = 20.0 nM 7.70
CHEMBL4102320 Kd = 43.0 nM 7.37
CHEMBL4100850 Kd = 61.0 nM 7.21
CHEMBL4071358 Kd = 100.0 nM 7.00
CHEMBL4072980 Kd = 140.0 nM 6.85
CHEMBL4075197 Kd = 150.0 nM 6.82
CHEMBL4077899 Kd = 200.0 nM 6.70
CHEMBL4078052 Kd = 210.0 nM 6.68
CHEMBL4092154 Kd = 290.0 nM 6.54
CHEMBL4079004 Kd = 300.0 nM 6.52
CHEMBL4093678 Kd = 1400.0 nM 5.85
CHEMBL4103307 Kd = 2600.0 nM 5.58
CHEMBL4067115 Kd = 3100.0 nM 5.51
CHEMBL4085375 Kd = 4800.0 nM 5.32
CHEMBL4093134 Kd = 9800.0 nM 5.01
CHEMBL4074086 Kd = 13400.0 nM 4.87
CHEMBL51594 Kd = 22000.0 nM 4.66
CHEMBL4074159 Kd = 38000.0 nM 4.42