Assay Detail
Binding
CHEMBL4020043
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to His tagged human EED (77 to 441 residues) expressed in Escherichia coli BL21(DE3)-T1R by ITC method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Are We There Yet? Applying Thermodynamic and Kinetic Profiling on Embryonic Ectoderm Development (EED) Hit-to-Lead Program.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 13 | 6.64 | 8.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4085428 | — | — | 1 | 8.22 |
| CHEMBL4084398 | — | — | 1 | 8.00 |
| CHEMBL4080018 | — | — | 1 | 8.00 |
| CHEMBL4072980 | — | — | 1 | 7.52 |
| CHEMBL4075197 | — | — | 1 | 7.05 |
| CHEMBL4077899 | — | — | 1 | 6.96 |
| CHEMBL4067115 | — | — | 1 | 6.43 |
| CHEMBL4093678 | — | — | 1 | 6.03 |
| CHEMBL4071358 | — | — | 1 | 5.94 |
| CHEMBL4060827 | — | — | 1 | 5.68 |
| CHEMBL4092154 | — | — | 1 | 5.66 |
| CHEMBL4064198 | — | — | 1 | 5.60 |
| CHEMBL51594 | — | — | 1 | 5.28 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4085428 | — | Kd | = | 6.0 | nM | 8.22 |
| CHEMBL4084398 | — | Kd | = | 10.0 | nM | 8.00 |
| CHEMBL4080018 | — | Kd | = | 10.0 | nM | 8.00 |
| CHEMBL4072980 | — | Kd | = | 30.0 | nM | 7.52 |
| CHEMBL4075197 | — | Kd | = | 90.0 | nM | 7.05 |
| CHEMBL4077899 | — | Kd | = | 110.0 | nM | 6.96 |
| CHEMBL4067115 | — | Kd | = | 370.0 | nM | 6.43 |
| CHEMBL4093678 | — | Kd | = | 940.0 | nM | 6.03 |
| CHEMBL4071358 | — | Kd | = | 1140.0 | nM | 5.94 |
| CHEMBL4060827 | — | Kd | = | 2100.0 | nM | 5.68 |
| CHEMBL4092154 | — | Kd | = | 2200.0 | nM | 5.66 |
| CHEMBL4064198 | — | Kd | = | 2500.0 | nM | 5.60 |
| CHEMBL51594 | — | Kd | = | 5200.0 | nM | 5.28 |