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Assay Detail

CHEMBL4020060

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Binding
Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin-conjugating enzyme E2 T (CHEMBL4105763)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mind the Metal: A Fragment Library-Derived Zinc Impurity Binds the E2 Ubiquitin-Conjugating Enzyme Ube2T and Induces Structural Rearrangements.
Morreale FE, Testa A, Chaugule VK, Bortoluzzi A, Ciulli A, Walden H.
J Med Chem (2017) 60 : 8183 -8191
PubMed 28933844 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 4.78 4.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1200679 ZINC CHLORIDE 4.0 1 4.80
CHEMBL4077491 1 4.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1200679 ZINC CHLORIDE Kd = 15700.0 nM 4.80
CHEMBL4077491 Kd = 17700.0 nM 4.75