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Assay Detail

CHEMBL4020520

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type KIT (unknown origin) using biotinylated poly-Glu-Tyr as substrate preincubated for 30 mins followed by substrate addition in presence of ATP by TR-FRET assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mast/stem cell growth factor receptor Kit (CHEMBL1936)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors to Overcome Secondary Mutations in the Stem Cell Factor Receptor KIT.
Kaitsiotou H, Keul M, Hardick J, Mühlenberg T, Ketzer J, Ehrt C, Krüll J, Medda F, Koch O, Giordanetto F, Bauer S, Rauh D.
J Med Chem (2017) 60 : 8801 -8815
PubMed 28991465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 8.00 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL535 SUNITINIB 4.0 1 8.96
CHEMBL4093459 1 8.80
CHEMBL1171837 PONATINIB 4.0 1 8.77
CHEMBL4101206 1 8.72
CHEMBL4103644 1 8.70
CHEMBL4076193 1 8.68
CHEMBL1784625 1 8.68
CHEMBL4097771 1 8.64
CHEMBL4085713 1 8.60
CHEMBL4070641 1 8.59
CHEMBL4077510 1 8.48
CHEMBL4092478 1 8.48
CHEMBL4074329 1 8.46
CHEMBL4100222 1 8.40
CHEMBL4075321 1 8.24
CHEMBL4090058 1 8.21
CHEMBL1946170 REGORAFENIB 4.0 1 8.21
CHEMBL4062017 1 8.19
CHEMBL4092506 1 8.17
CHEMBL4079252 1 8.17
CHEMBL941 IMATINIB 4.0 1 7.79
CHEMBL4071683 1 7.00
CHEMBL4077028 1 6.92
CHEMBL4065711 1 6.74
CHEMBL4094827 1 6.64
CHEMBL4082210 1 6.62
CHEMBL4103510 1 6.25
CHEMBL4087133 1 5.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL535 SUNITINIB IC50 = 1.1 nM 8.96
CHEMBL4093459 IC50 = 1.6 nM 8.80
CHEMBL1171837 PONATINIB IC50 = 1.7 nM 8.77
CHEMBL4101206 IC50 = 1.9 nM 8.72
CHEMBL4103644 IC50 = 2.0 nM 8.70
CHEMBL4076193 IC50 = 2.1 nM 8.68
CHEMBL1784625 IC50 = 2.1 nM 8.68
CHEMBL4097771 IC50 = 2.3 nM 8.64
CHEMBL4085713 IC50 = 2.5 nM 8.60
CHEMBL4070641 IC50 = 2.6 nM 8.59
CHEMBL4077510 IC50 = 3.3 nM 8.48
CHEMBL4092478 IC50 = 3.3 nM 8.48
CHEMBL4074329 IC50 = 3.5 nM 8.46
CHEMBL4100222 IC50 = 4.0 nM 8.40
CHEMBL4075321 IC50 = 5.8 nM 8.24
CHEMBL4090058 IC50 = 6.2 nM 8.21
CHEMBL1946170 REGORAFENIB IC50 = 6.1 nM 8.21
CHEMBL4062017 IC50 = 6.5 nM 8.19
CHEMBL4092506 IC50 = 6.7 nM 8.17
CHEMBL4079252 IC50 = 6.8 nM 8.17
CHEMBL941 IMATINIB IC50 = 16.4 nM 7.79
CHEMBL4071683 IC50 = 100.3 nM 7.00
CHEMBL4077028 IC50 = 120.9 nM 6.92
CHEMBL4065711 IC50 = 183.7 nM 6.74
CHEMBL4094827 IC50 = 230.7 nM 6.64
CHEMBL4082210 IC50 = 239.6 nM 6.62
CHEMBL4103510 IC50 = 555.7 nM 6.25
CHEMBL4087133 IC50 = 1039.3 nM 5.98