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Assay Detail

CHEMBL4020950

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [20-3H]phorbol 12,13-dibutyrate from RasGRP1 (unknown origin) in the presence of porcine brain phosphatidylserine by scintillation counting
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAS guanyl-releasing protein 1 (CHEMBL5953)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Exploring the influence of indololactone structure on selectivity for binding to the C1 domains of PKCα, PKCε, and RasGRP.
Elhalem E, Donadío LG, Zhou X, Lewin NE, Garcia LC, Lai CC, Kelley JA, Peach ML, Blumberg PM, Comin MJ.
Bioorg Med Chem (2017) 25 : 2971 -2980
PubMed 28392275 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 7.46 9.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL519741 1 9.24
CHEMBL4097154 1 7.65
CHEMBL4070999 1 7.65
CHEMBL4075373 1 7.50
CHEMBL4069954 1 7.40
CHEMBL4099579 1 7.20
CHEMBL4081576 1 6.88
CHEMBL4066764 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL519741 Ki = 0.58 nM 9.24
CHEMBL4097154 Ki = 22.6 nM 7.65
CHEMBL4070999 Ki = 22.6 nM 7.65
CHEMBL4075373 Ki = 31.3 nM 7.50
CHEMBL4069954 Ki = 40.0 nM 7.40
CHEMBL4099579 Ki = 63.3 nM 7.20
CHEMBL4081576 Ki = 133.0 nM 6.88
CHEMBL4066764 Ki = 666.0 nM 6.18