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Assay Detail

CHEMBL4022065

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His6-tagged PARP5a catalytic domain (1091 to 1325 residues) expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay
16
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase tankyrase-1 (CHEMBL6164)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of potent inhibitors of the mono(ADP-ribosyl)transferase, PARP14.
Upton K, Meyers M, Thorsell AG, Karlberg T, Holechek J, Lease R, Schey G, Wolf E, Lucente A, Schüler H, Ferraris D.
Bioorg Med Chem Lett (2017) 27 : 2907 -2911
PubMed 28495083 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.40 6.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4085694 2 6.24
CHEMBL4068366 2 6.05
CHEMBL4089522 2 5.57
CHEMBL4104503 2 5.32
CHEMBL4087082 2 5.22
CHEMBL4074448 2 5.10
CHEMBL4079213 2 5.03
CHEMBL4097232 2 5.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4085694 IC50 = 575.44 nM 6.24
CHEMBL4068366 IC50 = 900.0 nM 6.05
CHEMBL4068366 IC50 = 977.24 nM 6.01
CHEMBL4085694 IC50 = 2700.0 nM 5.57
CHEMBL4089522 IC50 = 2700.0 nM 5.57
CHEMBL4089522 IC50 = 2691.53 nM 5.57
CHEMBL4104503 IC50 = 4800.0 nM 5.32
CHEMBL4104503 IC50 = 4786.3 nM 5.32
CHEMBL4087082 IC50 = 6000.0 nM 5.22
CHEMBL4087082 IC50 = 6309.57 nM 5.20
CHEMBL4074448 IC50 = 8000.0 nM 5.10
CHEMBL4074448 IC50 = 7943.28 nM 5.10
CHEMBL4079213 IC50 = 9400.0 nM 5.03
CHEMBL4079213 IC50 = 9332.54 nM 5.03
CHEMBL4097232 IC50 = 9800.0 nM 5.01
CHEMBL4097232 IC50 = 9772.37 nM 5.01