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Assay Detail

CHEMBL4022894

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Corrector activity at HRP-fused CFTR F508del mutant (unknown origin) expressed in human CFBE41o cells assessed as increase in matured protein levels at cell surface after 18 to 24 hrs by luminescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cystic fibrosis transmembrane conductance regulator (CHEMBL4051)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 4-[(2R,4R)-4-({[1-(2,2-Difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl}amino)-7-(difluoromethoxy)-3,4-dihydro-2H-chromen-2-yl]benzoic Acid (ABBV/GLPG-2222), a Potent Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Corrector for the Treatment of Cystic Fibrosis.
Wang X, Liu B, Searle X, Yeung C, Bogdan A, Greszler S, Singh A, Fan Y, Swensen AM, Vortherms T, Balut C, Jia Y, Desino K, Gao W, Yong H, Tse C, Kym P.
J Med Chem (2018) 61 : 1436 -1449
PubMed 29251932 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 5.90 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4093612 A-1596586 1 6.89
CHEMBL4101344 1 6.70
CHEMBL4085871 1 6.64
CHEMBL4074654 1 6.35
CHEMBL4102835 1 6.03
CHEMBL4066040 A-1596584 1 6.00
CHEMBL4094248 1 5.80
CHEMBL4083915 1 5.64
CHEMBL4086410 1 5.60
CHEMBL4073541 1 5.60
CHEMBL4091682 1 5.57
CHEMBL4078555 1 5.41
CHEMBL4094173 1 5.37
CHEMBL4064946 1 4.96
CHEMBL4101906 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4093612 A-1596586 EC50 = 130.0 nM 6.89
CHEMBL4101344 EC50 = 200.0 nM 6.70
CHEMBL4085871 EC50 = 230.0 nM 6.64
CHEMBL4074654 EC50 = 450.0 nM 6.35
CHEMBL4102835 EC50 = 940.0 nM 6.03
CHEMBL4066040 A-1596584 EC50 = 1000.0 nM 6.00
CHEMBL4094248 EC50 = 1600.0 nM 5.80
CHEMBL4083915 EC50 = 2300.0 nM 5.64
CHEMBL4086410 EC50 = 2500.0 nM 5.60
CHEMBL4073541 EC50 = 2500.0 nM 5.60
CHEMBL4091682 EC50 = 2700.0 nM 5.57
CHEMBL4078555 EC50 = 3900.0 nM 5.41
CHEMBL4094173 EC50 = 4300.0 nM 5.37
CHEMBL4064946 EC50 = 11000.0 nM 4.96
CHEMBL4101906 EC50 > 20000.0 nM -