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Assay Detail

CHEMBL4022897

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Corrector activity at CFTR F508del/F508del mutant in primary HBE cells assessed as increase in chloride ion current across apical membrane measured 18 to 24 hrs post compound treatment on basolateral side of cells in presence of channel potentiator GLPG1837 by trans-epithelial current clamp method
10
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cystic fibrosis transmembrane conductance regulator (CHEMBL4051)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 4-[(2R,4R)-4-({[1-(2,2-Difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl}amino)-7-(difluoromethoxy)-3,4-dihydro-2H-chromen-2-yl]benzoic Acid (ABBV/GLPG-2222), a Potent Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Corrector for the Treatment of Cystic Fibrosis.
Wang X, Liu B, Searle X, Yeung C, Bogdan A, Greszler S, Singh A, Fan Y, Swensen AM, Vortherms T, Balut C, Jia Y, Desino K, Gao W, Yong H, Tse C, Kym P.
J Med Chem (2018) 61 : 1436 -1449
PubMed 29251932 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 7.16 8.30
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4101487 GALICAFTOR 2.0 1 8.30
CHEMBL4066196 1 8.05
CHEMBL4093612 A-1596586 1 7.55
CHEMBL4093771 1 7.12
CHEMBL4067042 1 7.06
CHEMBL4077999 1 6.96
CHEMBL4096042 1 6.68
CHEMBL4085871 1 6.62
CHEMBL4094248 1 6.12
CHEMBL2103870 LUMACAFTOR 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4101487 GALICAFTOR EC50 = 5.0 nM 8.30
CHEMBL4066196 EC50 = 9.0 nM 8.05
CHEMBL4093612 A-1596586 EC50 = 28.0 nM 7.55
CHEMBL4093771 EC50 = 75.0 nM 7.12
CHEMBL4067042 EC50 = 87.0 nM 7.06
CHEMBL4077999 EC50 = 110.0 nM 6.96
CHEMBL4096042 EC50 = 210.0 nM 6.68
CHEMBL4085871 EC50 = 240.0 nM 6.62
CHEMBL4094248 EC50 = 750.0 nM 6.12
CHEMBL2103870 LUMACAFTOR Activity - -