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Assay Detail

CHEMBL4023752

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK in human whole blood derived-basophils assessed as suppression of IgE mediated-FcepsilonR ligation-stimulated CD63 expression
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of GDC-0853: A Potent, Selective, and Noncovalent Bruton's Tyrosine Kinase Inhibitor in Early Clinical Development.
Crawford JJ, Johnson AR, Misner DL, Belmont LD, Castanedo G, Choy R, Coraggio M, Dong L, Eigenbrot C, Erickson R, Ghilardi N, Hau J, Katewa A, Kohli PB, Lee W, Lubach JW, McKenzie BS, Ortwine DF, Schutt L, Tay S, Wei B, Reif K, Liu L, Wong H, Young WB.
J Med Chem (2018) 61 : 2227 -2245
PubMed 29457982 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.75 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4065122 FENEBRUTINIB 3.0 1 7.51
CHEMBL1873475 IBRUTINIB 4.0 1 6.77
CHEMBL3707348 ACALABRUTINIB 4.0 1 6.53
CHEMBL4071161 TIRABRUTINIB 4.0 1 6.50
CHEMBL3952795 1 6.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4065122 FENEBRUTINIB IC50 = 30.7 nM 7.51
CHEMBL1873475 IBRUTINIB IC50 = 171.0 nM 6.77
CHEMBL3707348 ACALABRUTINIB IC50 = 296.9 nM 6.53
CHEMBL4071161 TIRABRUTINIB IC50 = 312.3 nM 6.50
CHEMBL3952795 IC50 = 374.3 nM 6.43