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Assay Detail

CHEMBL4023905

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activity at GFP10-tagged human APJ receptor expressed in HEK293 cells assessed as induction of RlucII-tagged beta-arrestin-2 recruitment after 30 mins by BRET assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL1628481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Systematic Exploration of Macrocyclization in Apelin-13: Impact on Binding, Signaling, Stability, and Cardiovascular Effects.
Trân K, Murza A, Sainsily X, Coquerel D, Côté J, Belleville K, Haroune L, Longpré JM, Dumaine R, Salvail D, Lesur O, Auger-Messier M, Sarret P, Marsault É.
J Med Chem (2018) 61 : 2266 -2277
PubMed 29461833 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 6.82 7.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4077162 1 7.46
CHEMBL4076652 1 7.38
CHEMBL4094033 1 7.24
CHEMBL4072606 1 7.24
CHEMBL3184840 1 7.22
CHEMBL4076049 1 7.20
CHEMBL4075302 1 7.04
CHEMBL4083079 1 6.90
CHEMBL4093319 1 6.84
CHEMBL4100822 1 6.75
CHEMBL4083756 1 6.63
CHEMBL4076755 1 6.57
CHEMBL4072080 1 6.56
CHEMBL4078407 1 6.40
CHEMBL4101053 1 6.29
CHEMBL4087671 1 6.08
CHEMBL4076140 1 6.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4077162 EC50 = 35.0 nM 7.46
CHEMBL4076652 EC50 = 42.0 nM 7.38
CHEMBL4094033 EC50 = 57.0 nM 7.24
CHEMBL4072606 EC50 = 58.0 nM 7.24
CHEMBL3184840 EC50 = 60.0 nM 7.22
CHEMBL4076049 EC50 = 63.0 nM 7.20
CHEMBL4075302 EC50 = 91.0 nM 7.04
CHEMBL4083079 EC50 = 126.0 nM 6.90
CHEMBL4093319 EC50 = 145.0 nM 6.84
CHEMBL4100822 EC50 = 179.0 nM 6.75
CHEMBL4083756 EC50 = 232.0 nM 6.63
CHEMBL4076755 EC50 = 272.0 nM 6.57
CHEMBL4072080 EC50 = 274.0 nM 6.56
CHEMBL4078407 EC50 = 400.0 nM 6.40
CHEMBL4101053 EC50 = 512.0 nM 6.29
CHEMBL4087671 EC50 = 825.0 nM 6.08
CHEMBL4076140 EC50 = 857.0 nM 6.07