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Assay Detail

CHEMBL4024005

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Induction of necroptosis in mouse L929 cells assessed as increase in TNFalpha/zVAD-FMK-induced LDH release preincubated for 30 mins followed by TNFalpha/zVAD-FMK addition measured after 4 hrs by CytoTox 96 nonradioactive cytotoxicity assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L929
Confidence 1 — Organism
Curated By Autocuration

Target

L929 (CHEMBL612267)
Type CELL-LINE
Organism Mus musculus

Publication

Discovery of 7-Oxo-2,4,5,7-tetrahydro-6 H-pyrazolo[3,4- c]pyridine Derivatives as Potent, Orally Available, and Brain-Penetrating Receptor Interacting Protein 1 (RIP1) Kinase Inhibitors: Analysis of Structure-Kinetic Relationships.
Yoshikawa M, Saitoh M, Katoh T, Seki T, Bigi SV, Shimizu Y, Ishii T, Okai T, Kuno M, Hattori H, Watanabe E, Saikatendu KS, Zou H, Nakakariya M, Tatamiya T, Nakada Y, Yogo T.
J Med Chem (2018) 61 : 2384 -2409
PubMed 29485864 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.82 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4100398 1 7.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4100398 IC50 = 15.0 nM 7.82