Assay Detail
Functional
CHEMBL4026893
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation after 10 mins by FRET assay
6
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structure-Based Design and Discovery of New M2 Receptor Agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 5.82 | 7.31 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL667 | ACETYLCHOLINE | 4.0 | 2 | 7.31 |
| CHEMBL4091934 | — | — | 2 | 5.20 |
| CHEMBL4060823 | — | — | 2 | 4.96 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL667 | ACETYLCHOLINE | EC50 | = | 48.98 | nM | 7.31 |
| CHEMBL667 | ACETYLCHOLINE | EC50 | = | 49.0 | nM | 7.31 |
| CHEMBL4091934 | — | EC50 | = | 6309.57 | nM | 5.20 |
| CHEMBL4091934 | — | EC50 | = | 6300.0 | nM | 5.20 |
| CHEMBL4060823 | — | EC50 | = | 10964.78 | nM | 4.96 |
| CHEMBL4060823 | — | EC50 | = | 11000.0 | nM | 4.96 |