Assay Detail
Binding
CHEMBL4026899
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at recombinant human muscarinic M2 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay
6
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structure-Based Design and Discovery of New M2 Receptor Agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 6.87 | 7.90 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL667 | ACETYLCHOLINE | 4.0 | 2 | 7.90 |
| CHEMBL4060823 | — | — | 2 | 6.62 |
| CHEMBL4091934 | — | — | 2 | 6.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL667 | ACETYLCHOLINE | EC50 | = | 12.59 | nM | 7.90 |
| CHEMBL667 | ACETYLCHOLINE | EC50 | = | 13.0 | nM | 7.89 |
| CHEMBL4060823 | — | EC50 | = | 239.88 | nM | 6.62 |
| CHEMBL4060823 | — | EC50 | = | 240.0 | nM | 6.62 |
| CHEMBL4091934 | — | EC50 | = | 831.76 | nM | 6.08 |
| CHEMBL4091934 | — | EC50 | = | 830.0 | nM | 6.08 |