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Assay Detail

CHEMBL4026937

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Potentiation of CFTR F508del mutant (unknown origin) expressed in human CFBE41o cells incubated for 10 mins in presence of forskolin measured for 7 secs by YFP halide assay
45
Total Activities
28
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cystic fibrosis transmembrane conductance regulator (CHEMBL4051)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-(3-Carbamoyl-5,5,7,7-tetramethyl-5,7-dihydro-4H-thieno[2,3-c]pyran-2-yl)-lH-pyrazole-5-carboxamide (GLPG1837), a Novel Potentiator Which Can Open Class III Mutant Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Channels to a High Extent.
Van der Plas SE, Kelgtermans H, De Munck T, Martina SLX, Dropsit S, Quinton E, De Blieck A, Joannesse C, Tomaskovic L, Jans M, Christophe T, van der Aar E, Borgonovi M, Nelles L, Gees M, Stouten P, Van Der Schueren J, Mammoliti O, Conrath K, Andrews M.
J Med Chem (2018) 61 : 1425 -1435
PubMed 29148763 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 7.56 9.27
Activity 18 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4084934 2 9.27
CHEMBL4087529 2 8.70
CHEMBL4066158 2 8.70
CHEMBL4102980 2 8.57
CHEMBL4103906 2 8.52
CHEMBL4075348 2 8.52
CHEMBL4059736 2 8.40
CHEMBL4104967 2 8.40
CHEMBL4076873 2 7.96
CHEMBL4079652 2 7.89
CHEMBL4087115 2 7.89
CHEMBL4083374 2 7.82
CHEMBL4101361 1 7.80
CHEMBL4077923 1 7.64
CHEMBL4085032 1 7.12
CHEMBL4082311 1 7.00
CHEMBL2010601 IVACAFTOR 4.0 2 6.90
CHEMBL4067101 1 6.90
CHEMBL4077574 2 6.90
CHEMBL4059537 1 6.87
CHEMBL4066461 1 6.83
CHEMBL4081673 1 6.72
CHEMBL210832 2 6.49
CHEMBL4076611 1 6.33
CHEMBL4078274 2 6.19
CHEMBL4097262 2 6.11
CHEMBL4101451 1 -
CHEMBL4079238 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4084934 EC50 = 0.54 nM 9.27
CHEMBL4087529 EC50 = 2.0 nM 8.70
CHEMBL4066158 EC50 = 2.0 nM 8.70
CHEMBL4102980 EC50 = 2.7 nM 8.57
CHEMBL4103906 EC50 = 3.0 nM 8.52
CHEMBL4075348 EC50 = 3.0 nM 8.52
CHEMBL4104967 EC50 = 4.0 nM 8.40
CHEMBL4059736 EC50 = 4.0 nM 8.40
CHEMBL4076873 EC50 = 11.0 nM 7.96
CHEMBL4079652 EC50 = 13.0 nM 7.89
CHEMBL4087115 EC50 = 13.0 nM 7.89
CHEMBL4083374 EC50 = 15.0 nM 7.82
CHEMBL4101361 EC50 = 16.0 nM 7.80
CHEMBL4077923 EC50 = 23.0 nM 7.64
CHEMBL4085032 EC50 = 75.0 nM 7.12
CHEMBL4082311 EC50 = 101.0 nM 7.00
CHEMBL4067101 EC50 = 125.0 nM 6.90
CHEMBL4077574 EC50 = 127.0 nM 6.90
CHEMBL2010601 IVACAFTOR EC50 = 126.0 nM 6.90
CHEMBL4059537 EC50 = 136.0 nM 6.87
CHEMBL4066461 EC50 = 147.0 nM 6.83
CHEMBL4081673 EC50 = 190.0 nM 6.72
CHEMBL210832 EC50 = 326.0 nM 6.49
CHEMBL4076611 EC50 = 467.0 nM 6.33
CHEMBL4078274 EC50 = 652.0 nM 6.19
CHEMBL4097262 EC50 = 778.0 nM 6.11
CHEMBL4101451 EC50 > 10000.0 nM -
CHEMBL4079238 Activity = 3.0 % -
CHEMBL210832 Activity = 71.0 % -
CHEMBL4078274 Activity = 72.0 % -
CHEMBL4097262 Activity = 74.0 % -
CHEMBL4087115 Activity = 93.0 % -
CHEMBL4104967 Activity = 98.0 % -
CHEMBL4059736 Activity = 111.0 % -
CHEMBL4075348 Activity = 105.0 % -
CHEMBL4076873 Activity = 92.0 % -
CHEMBL4079652 Activity = 81.0 % -
CHEMBL4103906 Activity = 107.0 % -
CHEMBL4087529 Activity = 96.0 % -
CHEMBL4066158 Activity = 96.0 % -
CHEMBL2010601 IVACAFTOR Activity = 100.0 % -
CHEMBL4102980 Activity = 95.0 % -
CHEMBL4084934 Activity = 102.0 % -
CHEMBL4077574 Activity = 95.0 % -
CHEMBL4083374 Activity = 105.0 % -