Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4026983

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IRAK4 in human KARPAS299 cells assessed as reduction in IL-1 stimulated IRAK4 phosphorylation at Thr345/Ser346 residues preincubated for 1 hr followed by IL-1 stimulation for 10 mins by flow cytometry analysis
38
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Interleukin-1 receptor-associated kinase 4 (CHEMBL3778)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Optimization of Pyrrolopyrimidine Inhibitors of Interleukin-1 Receptor Associated Kinase 4 (IRAK4) for the Treatment of Mutant MYD88L265P Diffuse Large B-Cell Lymphoma.
Scott JS, Degorce SL, Anjum R, Culshaw J, Davies RDM, Davies NL, Dillman KS, Dowling JE, Drew L, Ferguson AD, Groombridge SD, Halsall CT, Hudson JA, Lamont S, Lindsay NA, Marden SK, Mayo MF, Pease JE, Perkins DR, Pink JH, Robb GR, Rosen A, Shen M, McWhirter C, Wu D.
J Med Chem (2017) 60 : 10071 -10091
PubMed 29172502 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 38 6.58 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4097418 1 7.64
CHEMBL4085735 1 7.54
CHEMBL4104802 1 7.47
CHEMBL4081502 2 7.47
CHEMBL4087063 1 7.46
CHEMBL4078222 1 7.42
CHEMBL4078625 1 7.38
CHEMBL4102501 1 7.38
CHEMBL4064608 1 7.28
CHEMBL4080574 1 7.24
CHEMBL4089379 1 7.23
CHEMBL4077215 1 7.20
CHEMBL4061227 1 7.18
CHEMBL4091013 1 7.12
CHEMBL4067181 1 7.09
CHEMBL4088438 1 6.92
CHEMBL4063689 1 6.85
CHEMBL4067302 1 6.80
CHEMBL4063127 1 6.60
CHEMBL4086115 1 6.57
CHEMBL4097089 1 6.55
CHEMBL4079194 1 6.52
CHEMBL4104915 1 6.47
CHEMBL4069890 1 6.40
CHEMBL3361252 1 6.31
CHEMBL4064168 1 6.09
CHEMBL4075333 2 5.92
CHEMBL4093475 1 5.85
CHEMBL4087705 1 5.77
CHEMBL4069060 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4097418 IC50 = 23.0 nM 7.64
CHEMBL4085735 IC50 = 29.0 nM 7.54
CHEMBL4081502 IC50 = 34.0 nM 7.47
CHEMBL4104802 IC50 = 34.0 nM 7.47
CHEMBL4087063 IC50 = 35.0 nM 7.46
CHEMBL4078222 IC50 = 38.0 nM 7.42
CHEMBL4102501 IC50 = 42.0 nM 7.38
CHEMBL4078625 IC50 = 42.0 nM 7.38
CHEMBL4064608 IC50 = 52.0 nM 7.28
CHEMBL4080574 IC50 = 58.0 nM 7.24
CHEMBL4089379 IC50 = 59.0 nM 7.23
CHEMBL4077215 IC50 = 63.0 nM 7.20
CHEMBL4061227 IC50 = 66.0 nM 7.18
CHEMBL4091013 IC50 = 75.0 nM 7.12
CHEMBL4067181 IC50 = 82.0 nM 7.09
CHEMBL4088438 IC50 = 120.0 nM 6.92
CHEMBL4063689 IC50 = 140.0 nM 6.85
CHEMBL4067302 IC50 = 160.0 nM 6.80
CHEMBL4063127 IC50 = 250.0 nM 6.60
CHEMBL4086115 IC50 = 270.0 nM 6.57
CHEMBL4097089 IC50 = 280.0 nM 6.55
CHEMBL4079194 IC50 = 300.0 nM 6.52
CHEMBL4104915 IC50 = 340.0 nM 6.47
CHEMBL4069890 IC50 = 400.0 nM 6.40
CHEMBL3361252 IC50 = 490.0 nM 6.31
CHEMBL4081502 IC50 = 540.0 nM 6.27
CHEMBL4064168 IC50 = 810.0 nM 6.09
CHEMBL4075333 IC50 = 1200.0 nM 5.92
CHEMBL4093475 IC50 = 1400.0 nM 5.85
CHEMBL4087705 IC50 = 1700.0 nM 5.77
CHEMBL4075333 IC50 = 2100.0 nM 5.68
CHEMBL4069060 IC50 = 2500.0 nM 5.60
CHEMBL4094760 IC50 = 3100.0 nM 5.51
CHEMBL4096351 IC50 = 3600.0 nM 5.44
CHEMBL4079819 IC50 = 4300.0 nM 5.37
CHEMBL4097847 IC50 = 8400.0 nM 5.08
CHEMBL4105582 IC50 = 23000.0 nM 4.64
CHEMBL4086053 IC50 > 30000.0 nM -