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Assay Detail

CHEMBL4028015

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human N-terminal FLAG-tagged PDE2A3 expressed in sf21 cells using [3H]cGMP as substrate after 30 mins by SPA
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-dependent 3',5'-cyclic phosphodiesterase (CHEMBL2652)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a Potent, Highly Selective, and Brain Penetrant Phosphodiesterase 2A Inhibitor Clinical Candidate.
Helal CJ, Arnold E, Boyden T, Chang C, Chappie TA, Fisher E, Hajos M, Harms JF, Hoffman WE, Humphrey JM, Pandit J, Kang Z, Kleiman RJ, Kormos BL, Lee CW, Lu J, Maklad N, McDowell L, McGinnis D, O'Connor RE, O'Donnell CJ, Ogden A, Piotrowski M, Schmidt CJ, Seymour PA, Ueno H, Vansell N, Verhoest PR, Yang EX.
J Med Chem (2018) 61 : 1001 -1018
PubMed 29293004 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 8.35 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3655786 1 9.52
CHEMBL4080109 1 8.89
CHEMBL2381192 1 8.80
CHEMBL3092562 PF-05180999 1.0 1 8.80
CHEMBL3655780 1 8.09
CHEMBL3092561 1 8.05
CHEMBL4081035 1 7.96
CHEMBL4086493 1 7.92
CHEMBL4099071 1 7.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3655786 IC50 = 0.3 nM 9.52
CHEMBL4080109 IC50 = 1.3 nM 8.89
CHEMBL2381192 IC50 = 1.6 nM 8.80
CHEMBL3092562 PF-05180999 IC50 = 1.6 nM 8.80
CHEMBL3655780 IC50 = 8.2 nM 8.09
CHEMBL3092561 IC50 = 9.0 nM 8.05
CHEMBL4081035 IC50 = 11.0 nM 7.96
CHEMBL4086493 IC50 = 12.0 nM 7.92
CHEMBL4099071 IC50 = 72.0 nM 7.14