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Assay Detail

CHEMBL4028398

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Binding
Inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) using nicotinamide/SAM as substrate/co-factor measured for 20 mins by SAHH-coupled fluorescent assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide N-methyltransferase (CHEMBL2346486)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Bisubstrate Inhibitors of Nicotinamide N-Methyltransferase (NNMT).
Babault N, Allali-Hassani A, Li F, Fan J, Yue A, Ju K, Liu F, Vedadi M, Liu J, Jin J.
J Med Chem (2018) 61 : 1541 -1551
PubMed 29320176 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 4.85 4.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4067973 1 4.85
CHEMBL4100002 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4067973 IC50 = 14000.0 nM 4.85
CHEMBL4100002 IC50 = 160000.0 nM -