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Assay Detail

CHEMBL4029995

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 1 hr followed by LPS stimulation measured after 24 hrs by Griess assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

New derivatives of ursolic acid through the biotransformation by Bacillus megaterium CGMCC 1.1741 as inhibitors on nitric oxide production.
Zhang C, Xu SH, Ma BL, Wang WW, Yu BY, Zhang J.
Bioorg Med Chem Lett (2017) 27 : 2575 -2578
PubMed 28427811 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.35 5.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4074068 1 5.91
CHEMBL4094602 1 5.77
CHEMBL50 QUERCETIN 3.0 1 5.36
CHEMBL487887 URSONIC ACID 1 5.31
CHEMBL4065446 1 4.40
CHEMBL4086892 1 -
CHEMBL169 URSOLIC ACID 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4074068 IC50 = 1240.0 nM 5.91
CHEMBL4094602 IC50 = 1710.0 nM 5.77
CHEMBL50 QUERCETIN IC50 = 4410.0 nM 5.36
CHEMBL487887 URSONIC ACID IC50 = 4940.0 nM 5.31
CHEMBL4065446 IC50 = 40090.0 nM 4.40
CHEMBL4086892 IC50 > 60000.0 nM -
CHEMBL169 URSOLIC ACID IC50 - -