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Assay Detail

CHEMBL4031182

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of cGAS in human THP1 cells assessed as reduction in salmon sperm dsDNA-induced IFN-beta expression preincubated for 1 hr followed by dsDNA stimulation for 12 hrs by luciferase reporter gene assay
5
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclic GMP-AMP synthase (CHEMBL4105728)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of PF-06928215 as a high affinity inhibitor of cGAS enabled by a novel fluorescence polarization assay.
Hall J, Brault A, Vincent F, Weng S, Wang H, Dumlao D, Aulabaugh A, Aivazian D, Castro D, Chen M, Culp J, Dower K, Gardner J, Hawrylik S, Golenbock D, Hepworth D, Horn M, Jones L, Jones P, Latz E, Li J, Lin LL, Lin W, Lin D, Lovering F, Niljanskul N, Nistler R, Pierce B, Plotnikova O, Schmitt D, Shanker S, Smith J, Snyder W, Subashi T, Trujillo J, Tyminski E, Wang G, Wong J, Lefker B, Dakin L, Leach K.
PLoS One (2017) 12 : e0184843 -null
PubMed 28934246 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 4 - -
IC50 1 7.22 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL577784 BX-795 1 7.22
CHEMBL4096573 1 -
CHEMBL4084664 1 -
CHEMBL4062994 1 -
CHEMBL4103560 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL577784 BX-795 IC50 = 60.0 nM 7.22
CHEMBL4096573 Inhibition - % -
CHEMBL4084664 Inhibition - % -
CHEMBL4062994 Inhibition - % -
CHEMBL4103560 Inhibition - % -