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Assay Detail

CHEMBL4031825

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human wild type LRRK2 phosphorylation at ser935 expressed in BacMam transduced HEK293 cells after 48 hrs by in-cell Western assay
14
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design of Leucine-Rich Repeat Kinase 2 (LRRK2) Inhibitors Using a Crystallographic Surrogate Derived from Checkpoint Kinase 1 (CHK1).
Williamson DS, Smith GP, Acheson-Dossang P, Bedford ST, Chell V, Chen IJ, Daechsel JCA, Daniels Z, David L, Dokurno P, Hentzer M, Herzig MC, Hubbard RE, Moore JD, Murray JB, Newland S, Ray SC, Shaw T, Surgenor AE, Terry L, Thirstrup K, Wang Y, Christensen KV.
J Med Chem (2017) 60 : 8945 -8962
PubMed 29023112 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.88 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3969077 1 7.77
CHEMBL3961632 2 7.17
CHEMBL3933899 1 7.16
CHEMBL4084769 1 7.12
CHEMBL3939513 1 7.07
CHEMBL4100265 1 7.03
CHEMBL4092521 1 6.96
CHEMBL3961481 1 6.89
CHEMBL4101218 1 6.80
CHEMBL3919862 1 6.24
CHEMBL4063115 1 6.16
CHEMBL4077034 1 5.89
CHEMBL4065198 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3969077 IC50 = 17.0 nM 7.77
CHEMBL3961632 IC50 = 67.0 nM 7.17
CHEMBL3961632 IC50 = 67.61 nM 7.17
CHEMBL3933899 IC50 = 70.0 nM 7.16
CHEMBL4084769 IC50 = 76.0 nM 7.12
CHEMBL3939513 IC50 = 85.0 nM 7.07
CHEMBL4100265 IC50 = 93.0 nM 7.03
CHEMBL4092521 IC50 = 110.0 nM 6.96
CHEMBL3961481 IC50 = 130.0 nM 6.89
CHEMBL4101218 IC50 = 160.0 nM 6.80
CHEMBL3919862 IC50 = 580.0 nM 6.24
CHEMBL4063115 IC50 = 700.0 nM 6.16
CHEMBL4077034 IC50 = 1300.0 nM 5.89
CHEMBL4065198 IC50 - -