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Assay Detail

CHEMBL4035336

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CYP1B1 in human liver microsomes coexpressing recombinant human cytochrome P450 oxidoreductase using 7-ethoxyresorufin as substrate after 3 mins in presence of NADP+ by fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1B1 (CHEMBL4878)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of cytochrome P450 (CYP) 1B1.
Dutour R, Poirier D.
Eur J Med Chem (2017) 135 : 296 -306
PubMed 28458135 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 5.58 5.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL131921 BIOCHANIN 1 5.72
CHEMBL242341 FORMONONETIN 2.0 1 5.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL131921 BIOCHANIN Ki = 1900.0 nM 5.72
CHEMBL242341 FORMONONETIN Ki = 3700.0 nM 5.43