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Assay Detail

CHEMBL4035350

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human CYP1B1 expressed in Escherichia coli DH5alpha coexpressing human NADPH-P450 reductase using 4-estradiol as substrate in presence of NADP+ by HPLC analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1B1 (CHEMBL4878)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of cytochrome P450 (CYP) 1B1.
Dutour R, Poirier D.
Eur J Med Chem (2017) 135 : 296 -306
PubMed 28458135 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.62 6.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL295124 BERBERINE 4.0 1 6.48
CHEMBL1187148 THALIDENDINE 1 5.39
CHEMBL379449 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL295124 BERBERINE IC50 = 330.0 nM 6.48
CHEMBL1187148 THALIDENDINE IC50 = 4100.0 nM 5.39
CHEMBL379449 IC50 = 9900.0 nM 5.00