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Assay Detail

CHEMBL4035843

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of KDM5A in human PC9 cells assessed as increase in H3K4me3 level after 5 days by mass-spectrometric method
14
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 5A (CHEMBL2424504)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

From a novel HTS hit to potent, selective, and orally bioavailable KDM5 inhibitors.
Liang J, Labadie S, Zhang B, Ortwine DF, Patel S, Vinogradova M, Kiefer JR, Mauer T, Gehling VS, Harmange JC, Cummings R, Lai T, Liao J, Zheng X, Liu Y, Gustafson A, Van der Porten E, Mao W, Liederer BM, Deshmukh G, An L, Ran Y, Classon M, Trojer P, Dragovich PS, Murray L.
Bioorg Med Chem Lett (2017) 27 : 2974 -2981
PubMed 28512031 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 5.71 6.02
IC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4088737 1 6.02
CHEMBL4059597 3 5.82
CHEMBL4060968 2 5.80
CHEMBL4071387 1 5.19
CHEMBL4083572 3 -
CHEMBL4099924 1 -
CHEMBL4081928 1 -
CHEMBL4061676 1 -
CHEMBL4089789 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4088737 EC50 = 960.0 nM 6.02
CHEMBL4059597 EC50 = 1500.0 nM 5.82
CHEMBL4060968 EC50 = 1600.0 nM 5.80
CHEMBL4071387 EC50 = 6500.0 nM 5.19
CHEMBL4083572 EC50 > 30000.0 nM -
CHEMBL4083572 IC50 - -
CHEMBL4083572 IC50 > 30000.0 nM -
CHEMBL4099924 EC50 - -
CHEMBL4081928 EC50 - -
CHEMBL4061676 EC50 - -
CHEMBL4089789 EC50 - -
CHEMBL4059597 EC50 - -
CHEMBL4059597 EC50 - -
CHEMBL4060968 EC50 - -