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Assay Detail

CHEMBL4036010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of jack bean urease using urea as substrate pretreated for 15 mins followed by substrate addition by phenol red based spectrophotometric method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canavalia ensiformis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Urease (CHEMBL4161)
Type SINGLE PROTEIN
Organism Canavalia ensiformis

Publication

Synthesis and molecular docking study of some 5,6-dichloro-2-cyclopropyl-1H-benzimidazole derivatives bearing triazole, oxadiazole, and imine functionalities as potent inhibitors of urease.
Menteşe E, Bektaş H, Sokmen BB, Emirik M, Çakır D, Kahveci B.
Bioorg Med Chem Lett (2017) 27 : 3014 -3018
PubMed 28526368 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.45 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4100084 1 7.30
CHEMBL4092986 1 7.22
CHEMBL4071196 1 6.85
CHEMBL4092021 1 6.82
CHEMBL4070510 1 6.77
CHEMBL4103174 1 6.77
CHEMBL4065321 1 6.66
CHEMBL4085244 1 6.55
CHEMBL4100705 1 6.54
CHEMBL4074987 1 6.42
CHEMBL260876 THIOUREA 1 6.25
CHEMBL4083652 1 5.95
CHEMBL4073245 1 5.87
CHEMBL4073930 1 5.83
CHEMBL4061886 1 5.72
CHEMBL4095419 1 5.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4100084 IC50 = 50.0 nM 7.30
CHEMBL4092986 IC50 = 60.0 nM 7.22
CHEMBL4071196 IC50 = 140.0 nM 6.85
CHEMBL4092021 IC50 = 150.0 nM 6.82
CHEMBL4070510 IC50 = 170.0 nM 6.77
CHEMBL4103174 IC50 = 170.0 nM 6.77
CHEMBL4065321 IC50 = 220.0 nM 6.66
CHEMBL4085244 IC50 = 280.0 nM 6.55
CHEMBL4100705 IC50 = 290.0 nM 6.54
CHEMBL4074987 IC50 = 380.0 nM 6.42
CHEMBL260876 THIOUREA IC50 = 560.0 nM 6.25
CHEMBL4083652 IC50 = 1120.0 nM 5.95
CHEMBL4073245 IC50 = 1340.0 nM 5.87
CHEMBL4073930 IC50 = 1490.0 nM 5.83
CHEMBL4061886 IC50 = 1920.0 nM 5.72
CHEMBL4095419 IC50 = 2070.0 nM 5.68