Assay Detail
Binding
CHEMBL4036263
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human full length Cdc7 (1 to 574 residues)/human N-terminal GST-tagged ASK (1 to 674 residues) expressed in baculovirus expression system using N-terminal His-tagged MCM2 as substrate pretreated for 60 mins followed by 50 uM ATP addition by TR-FRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
CDC7/DBF4 (Cell division cycle 7-related protein kinase/Activator of S phase kinase) (CHEMBL2111377) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
2-Aminomethylthieno[3,2-d]pyrimidin-4(3H)-ones bearing 3-methylpyrazole hinge binding moiety: Highly potent, selective, and time-dependent inhibitors of Cdc7 kinase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 7.95 | 8.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4062209 | — | — | 1 | 8.82 |
| CHEMBL4083927 | — | — | 1 | 8.77 |
| CHEMBL4080673 | — | — | 1 | 8.25 |
| CHEMBL4098392 | — | — | 1 | 7.11 |
| CHEMBL4080367 | — | — | 1 | 6.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4062209 | — | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL4083927 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL4080673 | — | IC50 | = | 5.6 | nM | 8.25 |
| CHEMBL4098392 | — | IC50 | = | 77.0 | nM | 7.11 |
| CHEMBL4080367 | — | IC50 | = | 150.0 | nM | 6.82 |