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Assay Detail

CHEMBL4036454

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Agaricus bisporus tyrosinase assessed as reduction in diphenolase activity by measuring L-DOPA oxidation at 1.6 mM after 30 mins by Lineweaver-Burk plot
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Mushroom tyrosinase (CHEMBL6066154)
Type PROTEIN FAMILY
Organism Agaricus bisporus

Publication

Thiosemicarbazones as inhibitors of tyrosinase enzyme.
Soares MA, Almeida MA, Marins-Goulart C, Chaves OA, Echevarria A, de Oliveira MCC.
Bioorg Med Chem Lett (2017) 27 : 3546 -3550
PubMed 28583798 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 4.25 4.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL398206 1 4.30
CHEMBL3190453 1 4.22
CHEMBL242104 1 4.22
CHEMBL4105403 1 -
CHEMBL391982 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL398206 Ki = 50000.0 nM 4.30
CHEMBL3190453 Ki = 60000.0 nM 4.22
CHEMBL242104 Ki = 60000.0 nM 4.22
CHEMBL4105403 Ki = 200000.0 nM -
CHEMBL391982 Ki = 1300000.0 nM -