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Assay Detail

CHEMBL4036699

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length His-tagged BTK expressed in baculovirus expression system by Z-LYTE assay
21
Total Activities
20
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Selective Tricyclic Inhibitors of Bruton's Tyrosine Kinase with Improved Druglike Properties.
Wang X, Barbosa J, Blomgren P, Bremer MC, Chen J, Crawford JJ, Deng W, Dong L, Eigenbrot C, Gallion S, Hau J, Hu H, Johnson AR, Katewa A, Kropf JE, Lee SH, Liu L, Lubach JW, Macaluso J, Maciejewski P, Mitchell SA, Ortwine DF, DiPaolo J, Reif K, Scheerens H, Schmitt A, Wong H, Xiong JM, Xu J, Zhao Z, Zhou F, Currie KS, Young WB.
ACS Med Chem Lett (2017) 8 : 608 -613
PubMed 28626519 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.93 9.00
Ki 1 8.89 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3670081 1 9.00
CHEMBL3670082 2 8.89
CHEMBL3670094 1 8.70
CHEMBL4090189 1 8.70
CHEMBL4067714 1 8.52
CHEMBL4077625 1 8.52
CHEMBL4080943 1 8.52
CHEMBL4069030 1 8.40
CHEMBL3670139 1 8.40
CHEMBL3670088 1 8.40
CHEMBL3670095 1 8.40
CHEMBL4096404 1 8.30
CHEMBL3670086 1 8.22
CHEMBL4060453 1 8.10
CHEMBL4102294 1 7.96
CHEMBL4084331 1 7.38
CHEMBL4071845 1 7.09
CHEMBL4095477 1 6.86
CHEMBL4100365 1 5.58
CHEMBL4082346 1 4.78

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3670081 IC50 = 1.0 nM 9.00
CHEMBL3670082 Ki = 1.28 nM 8.89
CHEMBL3670094 IC50 = 2.0 nM 8.70
CHEMBL3670082 IC50 = 2.0 nM 8.70
CHEMBL4090189 IC50 = 2.0 nM 8.70
CHEMBL4067714 IC50 = 3.0 nM 8.52
CHEMBL4077625 IC50 = 3.0 nM 8.52
CHEMBL4080943 IC50 = 3.0 nM 8.52
CHEMBL3670139 IC50 = 4.0 nM 8.40
CHEMBL4069030 IC50 = 4.0 nM 8.40
CHEMBL3670088 IC50 = 4.0 nM 8.40
CHEMBL3670095 IC50 = 4.0 nM 8.40
CHEMBL4096404 IC50 = 5.0 nM 8.30
CHEMBL3670086 IC50 = 6.0 nM 8.22
CHEMBL4060453 IC50 = 8.0 nM 8.10
CHEMBL4102294 IC50 = 11.0 nM 7.96
CHEMBL4084331 IC50 = 42.0 nM 7.38
CHEMBL4071845 IC50 = 81.0 nM 7.09
CHEMBL4095477 IC50 = 139.0 nM 6.86
CHEMBL4100365 IC50 = 2600.0 nM 5.58
CHEMBL4082346 IC50 = 16700.0 nM 4.78