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Assay Detail

CHEMBL4039221

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP1A2 in human liver microsomes using tacrine as substrate preincubated for 5 mins followed by NADPH addition measured after 8 mins by LC/MS/MS analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1A2 (CHEMBL3356)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-(5-Fluoropyridin-2-yl)-6-methyl-4-(pyrimidin-5-yloxy)picolinamide (VU0424238): A Novel Negative Allosteric Modulator of Metabotropic Glutamate Receptor Subtype 5 Selected for Clinical Evaluation.
Felts AS, Rodriguez AL, Blobaum AL, Morrison RD, Bates BS, Thompson Gray A, Rook JM, Tantawy MN, Byers FW, Chang S, Venable DF, Luscombe VB, Tamagnan GD, Niswender CM, Daniels JS, Jones CK, Conn PJ, Lindsley CW, Emmitte KA.
J Med Chem (2017) 60 : 5072 -5085
PubMed 28530802 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.23 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4077672 1 7.00
CHEMBL2386849 1 6.70
CHEMBL4096818 1 6.70
CHEMBL4076771 1 6.52
CHEMBL4062697 1 6.40
CHEMBL4095685 1 6.22
CHEMBL2386772 1 6.05
CHEMBL4082854 1 6.05
CHEMBL2386850 1 6.00
CHEMBL4103707 1 5.62
CHEMBL4090661 1 5.22
CHEMBL2440659 1 -
CHEMBL3660237 1 -
CHEMBL4100830 1 -
CHEMBL2386771 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4077672 IC50 = 100.0 nM 7.00
CHEMBL2386849 IC50 = 200.0 nM 6.70
CHEMBL4096818 IC50 = 200.0 nM 6.70
CHEMBL4076771 IC50 = 300.0 nM 6.52
CHEMBL4062697 IC50 = 400.0 nM 6.40
CHEMBL4095685 IC50 = 600.0 nM 6.22
CHEMBL2386772 IC50 = 900.0 nM 6.05
CHEMBL4082854 IC50 = 900.0 nM 6.05
CHEMBL2386850 IC50 = 1000.0 nM 6.00
CHEMBL4103707 IC50 = 2400.0 nM 5.62
CHEMBL4090661 IC50 = 6000.0 nM 5.22
CHEMBL2440659 IC50 < 100.0 nM -
CHEMBL3660237 IC50 < 100.0 nM -
CHEMBL4100830 IC50 < 100.0 nM -
CHEMBL2386771 IC50 > 30000.0 nM -