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Assay Detail

CHEMBL4040031

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcupine (unknown origin) expressed in HEK293 cells after day 1 post treatment by Super-top flash reporter gene assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein-serine O-palmitoleoyltransferase porcupine (CHEMBL1255163)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Scaffold Hopping and Optimization of Maleimide Based Porcupine Inhibitors.
Ho SY, Alam J, Jeyaraj DA, Wang W, Lin GR, Ang SH, Tan ESW, Lee MA, Ke Z, Madan B, Virshup DM, Ding LJ, Manoharan V, Chew YS, Low CB, Pendharkar V, Sangthongpitag K, Hill J, Keller TH, Poulsen A.
J Med Chem (2017) 60 : 6678 -6692
PubMed 28671458 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.97 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4080208 1 9.40
CHEMBL4096728 1 9.00
CHEMBL4080021 1 9.00
CHEMBL4069295 1 8.22
CHEMBL4104447 1 8.10
CHEMBL4066589 1 8.05
CHEMBL4096529 1 7.96
CHEMBL4095609 1 7.96
CHEMBL4087906 1 7.92
CHEMBL4103356 1 7.92
CHEMBL4066796 1 7.92
CHEMBL4061069 1 7.82
CHEMBL4094309 1 7.82
CHEMBL4066798 1 7.62
CHEMBL4104257 1 7.54
CHEMBL4075204 1 7.54
CHEMBL4102043 1 7.38
CHEMBL4086549 1 7.17
CHEMBL4078705 1 7.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4080208 IC50 = 0.4 nM 9.40
CHEMBL4096728 IC50 = 1.0 nM 9.00
CHEMBL4080021 IC50 = 1.0 nM 9.00
CHEMBL4069295 IC50 = 6.0 nM 8.22
CHEMBL4104447 IC50 = 8.0 nM 8.10
CHEMBL4066589 IC50 = 9.0 nM 8.05
CHEMBL4096529 IC50 = 11.0 nM 7.96
CHEMBL4095609 IC50 = 11.0 nM 7.96
CHEMBL4087906 IC50 = 12.0 nM 7.92
CHEMBL4103356 IC50 = 12.0 nM 7.92
CHEMBL4066796 IC50 = 12.0 nM 7.92
CHEMBL4061069 IC50 = 15.0 nM 7.82
CHEMBL4094309 IC50 = 15.0 nM 7.82
CHEMBL4066798 IC50 = 24.0 nM 7.62
CHEMBL4104257 IC50 = 29.0 nM 7.54
CHEMBL4075204 IC50 = 29.0 nM 7.54
CHEMBL4102043 IC50 = 42.0 nM 7.38
CHEMBL4086549 IC50 = 67.0 nM 7.17
CHEMBL4078705 IC50 = 72.0 nM 7.14