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Assay Detail

CHEMBL4040378

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GRK2 expressed in HEK-B2 cells assessed as isoproterenol-stimulated cAMP accumulation preincubation for 20 mins followed by isoproterenol stimulation measured after 20 mins by cAMP ALPHA-screen assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-adrenergic receptor kinase 1 (CHEMBL4079)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Evaluation of the Highly Selective and Potent G-Protein-Coupled Receptor Kinase 2 (GRK2) Inhibitor for the Potential Treatment of Heart Failure.
Okawa T, Aramaki Y, Yamamoto M, Kobayashi T, Fukumoto S, Toyoda Y, Henta T, Hata A, Ikeda S, Kaneko M, Hoffman ID, Sang BC, Zou H, Kawamoto T.
J Med Chem (2017) 60 : 6942 -6990
PubMed 28699740 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 23 4.75 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4072828 1 5.82
CHEMBL4086046 1 5.52
CHEMBL4094447 1 5.13
CHEMBL1738877 1 5.00
CHEMBL4071398 1 4.92
CHEMBL4073882 1 4.64
CHEMBL4070100 1 4.58
CHEMBL4061438 1 4.48
CHEMBL4082775 1 4.39
CHEMBL4064232 1 4.35
CHEMBL4095659 1 4.12
CHEMBL4083276 1 4.06
CHEMBL1738878 1 -
CHEMBL4103972 1 -
CHEMBL4091972 1 -
CHEMBL4091067 1 -
CHEMBL4075992 1 -
CHEMBL4091573 1 -
CHEMBL4063774 1 -
CHEMBL4077447 1 -
CHEMBL4060973 1 -
CHEMBL4082853 1 -
CHEMBL4086619 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4072828 EC50 = 1500.0 nM 5.82
CHEMBL4086046 EC50 = 3000.0 nM 5.52
CHEMBL4094447 EC50 = 7400.0 nM 5.13
CHEMBL1738877 EC50 = 10000.0 nM 5.00
CHEMBL4071398 EC50 = 12000.0 nM 4.92
CHEMBL4073882 EC50 = 23000.0 nM 4.64
CHEMBL4070100 EC50 = 26000.0 nM 4.58
CHEMBL4061438 EC50 = 33000.0 nM 4.48
CHEMBL4082775 EC50 = 41000.0 nM 4.39
CHEMBL4064232 EC50 = 45000.0 nM 4.35
CHEMBL4095659 EC50 = 76000.0 nM 4.12
CHEMBL4083276 EC50 = 87000.0 nM 4.06
CHEMBL1738878 EC50 - -
CHEMBL4103972 EC50 > 30000.0 nM -
CHEMBL4091972 EC50 - -
CHEMBL4091067 EC50 > 30000.0 nM -
CHEMBL4075992 EC50 - -
CHEMBL4091573 EC50 = 220000.0 nM -
CHEMBL4063774 EC50 = 930000.0 nM -
CHEMBL4077447 EC50 = 120000.0 nM -
CHEMBL4060973 EC50 - -
CHEMBL4082853 EC50 - -
CHEMBL4086619 EC50 = 1900000.0 nM -