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Assay Detail

CHEMBL4040853

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [35S]TBPS from GABA-A receptor in Sprague-Dawley rat cerebral cortex cell membranes after 90 mins in presence of GABA by liquid scintillation counting
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Neuroactive Steroids. 2. 3α-Hydroxy-3β-methyl-21-(4-cyano-1H-pyrazol-1'-yl)-19-nor-5β-pregnan-20-one (SAGE-217): A Clinical Next Generation Neuroactive Steroid Positive Allosteric Modulator of the (γ-Aminobutyric Acid)A Receptor.
Martinez Botella G, Salituro FG, Harrison BL, Beresis RT, Bai Z, Blanco MJ, Belfort GM, Dai J, Loya CM, Ackley MA, Althaus AL, Grossman SJ, Hoffmann E, Doherty JJ, Robichaud AJ.
J Med Chem (2017) 60 : 7810 -7819
PubMed 28753313 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.57 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4105630 ZURANOLONE 4.0 1 8.15
CHEMBL4103751 1 8.10
CHEMBL3427532 1 7.96
CHEMBL4085062 1 7.89
CHEMBL4073780 1 7.89
CHEMBL4084134 1 7.80
CHEMBL4104908 1 7.77
CHEMBL4091891 1 7.75
CHEMBL4062424 1 7.68
CHEMBL207538 BREXANOLONE 4.0 1 7.66
CHEMBL4081622 1 7.55
CHEMBL3427530 1 7.52
CHEMBL4100583 1 7.44
CHEMBL4103198 1 7.35
CHEMBL4073679 1 7.31
CHEMBL4071062 1 7.19
CHEMBL4072311 1 7.11
CHEMBL4078922 1 7.10
CHEMBL4098519 1 6.60
CHEMBL4080678 1 -
CHEMBL4099623 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4105630 ZURANOLONE IC50 = 7.0 nM 8.15
CHEMBL4103751 IC50 = 8.0 nM 8.10
CHEMBL3427532 IC50 = 11.0 nM 7.96
CHEMBL4085062 IC50 = 13.0 nM 7.89
CHEMBL4073780 IC50 = 13.0 nM 7.89
CHEMBL4084134 IC50 = 16.0 nM 7.80
CHEMBL4104908 IC50 = 17.0 nM 7.77
CHEMBL4091891 IC50 = 18.0 nM 7.75
CHEMBL4062424 IC50 = 21.0 nM 7.68
CHEMBL207538 BREXANOLONE IC50 = 22.0 nM 7.66
CHEMBL4081622 IC50 = 28.0 nM 7.55
CHEMBL3427530 IC50 = 30.0 nM 7.52
CHEMBL4100583 IC50 = 36.0 nM 7.44
CHEMBL4103198 IC50 = 45.0 nM 7.35
CHEMBL4073679 IC50 = 49.0 nM 7.31
CHEMBL4071062 IC50 = 64.0 nM 7.19
CHEMBL4072311 IC50 = 78.0 nM 7.11
CHEMBL4078922 IC50 = 80.0 nM 7.10
CHEMBL4098519 IC50 = 251.0 nM 6.60
CHEMBL4080678 IC50 > 3000.0 nM -
CHEMBL4099623 IC50 > 3000.0 nM -