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Assay Detail

CHEMBL4040926

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant human His-tagged PDE2A3 expressed in fall armyworm Sf9 cells using [3H]cGMP as substrate preincubated for 30 mins followed by substrate addition after 60 mins by scintillation proximity assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-dependent 3',5'-cyclic phosphodiesterase (CHEMBL2652)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of an Orally Bioavailable, Brain-Penetrating, in Vivo Active Phosphodiesterase 2A Inhibitor Lead Series for the Treatment of Cognitive Disorders.
Mikami S, Sasaki S, Asano Y, Ujikawa O, Fukumoto S, Nakashima K, Oki H, Kamiguchi N, Imada H, Iwashita H, Taniguchi T.
J Med Chem (2017) 60 : 7658 -7676
PubMed 28759228 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.45 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4059831 1 7.62
CHEMBL4088107 1 7.28
CHEMBL4104510 1 6.31
CHEMBL4064136 1 6.00
CHEMBL4085701 1 5.85
CHEMBL4094364 1 5.66
CHEMBL4102110 1 5.44
CHEMBL4076518 1 5.42
CHEMBL4068494 1 5.21
CHEMBL4086618 1 5.16
CHEMBL4066865 1 4.80
CHEMBL4065234 1 4.68
CHEMBL4077849 1 4.64
CHEMBL4101192 1 4.55
CHEMBL4083192 1 4.31
CHEMBL4103591 1 4.28
CHEMBL4074901 1 -
CHEMBL4095339 1 -
CHEMBL4087643 1 -
CHEMBL4080230 1 -
CHEMBL4095833 1 -
CHEMBL4079320 1 -
CHEMBL4066287 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4059831 IC50 = 24.0 nM 7.62
CHEMBL4088107 IC50 = 53.0 nM 7.28
CHEMBL4104510 IC50 = 490.0 nM 6.31
CHEMBL4064136 IC50 = 1000.0 nM 6.00
CHEMBL4085701 IC50 = 1400.0 nM 5.85
CHEMBL4094364 IC50 = 2200.0 nM 5.66
CHEMBL4102110 IC50 = 3600.0 nM 5.44
CHEMBL4076518 IC50 = 3800.0 nM 5.42
CHEMBL4068494 IC50 = 6100.0 nM 5.21
CHEMBL4086618 IC50 = 7000.0 nM 5.16
CHEMBL4066865 IC50 = 16000.0 nM 4.80
CHEMBL4065234 IC50 = 21000.0 nM 4.68
CHEMBL4077849 IC50 = 23000.0 nM 4.64
CHEMBL4101192 IC50 = 28000.0 nM 4.55
CHEMBL4083192 IC50 = 49000.0 nM 4.31
CHEMBL4103591 IC50 = 52000.0 nM 4.28
CHEMBL4074901 IC50 > 100000.0 nM -
CHEMBL4095339 IC50 > 100000.0 nM -
CHEMBL4087643 IC50 > 100000.0 nM -
CHEMBL4080230 IC50 > 100000.0 nM -
CHEMBL4095833 IC50 > 100000.0 nM -
CHEMBL4079320 IC50 > 100000.0 nM -
CHEMBL4066287 IC50 > 100000.0 nM -