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Assay Detail

CHEMBL4041417

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HCC827 cells harboring EGFR-delE746_A750 mutant incubated for 96 hrs measured on day 5 by CellTiterGlo assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCC827
Confidence 1 — Organism
Curated By Autocuration

Target

HCC827 (CHEMBL4296422)
Type CELL-LINE
Organism Homo sapiens

Publication

Structure-Guided Development of Covalent and Mutant-Selective Pyrazolopyrimidines to Target T790M Drug Resistance in Epidermal Growth Factor Receptor.
Engel J, Smith S, Lategahn J, Tumbrink HL, Goebel L, Becker C, Hennes E, Keul M, Unger A, Müller H, Baumann M, Schultz-Fademrecht C, Günther G, Hengstler JG, Rauh D.
J Med Chem (2017) 60 : 7725 -7744
PubMed 28853575 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 30 6.20 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1229592 1 7.70
CHEMBL3545308 ROCILETINIB 3.0 1 7.35
CHEMBL4099713 1 7.16
CHEMBL4100655 1 7.16
CHEMBL4072299 1 7.16
CHEMBL4062509 1 7.10
CHEMBL4094959 1 7.05
CHEMBL4060919 1 6.82
CHEMBL2180203 1 6.80
CHEMBL4099254 1 6.51
CHEMBL4073960 1 6.37
CHEMBL1873475 IBRUTINIB 4.0 1 6.35
CHEMBL4095447 1 6.30
CHEMBL4091966 1 6.23
CHEMBL2180204 1 6.19
CHEMBL4082783 1 5.94
CHEMBL4073874 1 5.92
CHEMBL4071151 1 5.69
CHEMBL4075015 1 5.67
CHEMBL4069586 1 5.50
CHEMBL4101735 1 5.19
CHEMBL4094527 1 5.00
CHEMBL4084214 1 4.65
CHEMBL4063701 1 4.63
CHEMBL4103121 1 4.59
CHEMBL4090601 1 -
CHEMBL4103201 1 -
CHEMBL939 GEFITINIB 4.0 1 -
CHEMBL1173655 AFATINIB 4.0 1 -
CHEMBL3353410 OSIMERTINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1229592 EC50 = 20.0 nM 7.70
CHEMBL3545308 ROCILETINIB EC50 = 45.0 nM 7.35
CHEMBL4099713 EC50 = 70.0 nM 7.16
CHEMBL4100655 EC50 = 70.0 nM 7.16
CHEMBL4072299 EC50 = 70.0 nM 7.16
CHEMBL4062509 EC50 = 80.0 nM 7.10
CHEMBL4094959 EC50 = 90.0 nM 7.05
CHEMBL4060919 EC50 = 150.0 nM 6.82
CHEMBL2180203 EC50 = 160.0 nM 6.80
CHEMBL4099254 EC50 = 310.0 nM 6.51
CHEMBL4073960 EC50 = 430.0 nM 6.37
CHEMBL1873475 IBRUTINIB EC50 = 450.0 nM 6.35
CHEMBL4095447 EC50 = 500.0 nM 6.30
CHEMBL4091966 EC50 = 589.0 nM 6.23
CHEMBL2180204 EC50 = 650.0 nM 6.19
CHEMBL4082783 EC50 = 1150.0 nM 5.94
CHEMBL4073874 EC50 = 1190.0 nM 5.92
CHEMBL4071151 EC50 = 2050.0 nM 5.69
CHEMBL4075015 EC50 = 2130.0 nM 5.67
CHEMBL4069586 EC50 = 3150.0 nM 5.50
CHEMBL4101735 EC50 = 6390.0 nM 5.19
CHEMBL4094527 EC50 = 9900.0 nM 5.00
CHEMBL4084214 EC50 = 22300.0 nM 4.65
CHEMBL4063701 EC50 = 23600.0 nM 4.63
CHEMBL4103121 EC50 = 25900.0 nM 4.59
CHEMBL4090601 EC50 < 14.0 nM -
CHEMBL4103201 EC50 < 14.0 nM -
CHEMBL939 GEFITINIB EC50 < 14.0 nM -
CHEMBL1173655 AFATINIB EC50 < 14.0 nM -
CHEMBL3353410 OSIMERTINIB EC50 < 14.0 nM -